We narrowed to 15,153 results for: RING;

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pPRKCZ-C20-EGFP (C-PRKCZ-C20)

Plasmid

#217761

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Tags

EGFP

Expression

Mammalian

Mutation

C20 domain (aa 405-592)

Promoter

CMV

Available Since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

Plasmid

#217762

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Tags

EGFP

Expression

Mammalian

Mutation

Linker GGSSGGGA plus C20 domain (aa 405-592)

Promoter

CMV

Available Since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pC1_oROS-HT_LF(C199S)

Plasmid

#216412

Purpose

Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT_LF(C199S)

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP

Plasmid

#212109

Purpose

Expression of N-terminally tagged GABARAP tandem construct with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

mTagBFP2-GABARAP(G116A)-GABARAP (GABARAP Human, Entacmaea quadricolor)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_SAFBgRNA9

Plasmid

#196108

Purpose

Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_297-370

Plasmid

#196529

Purpose

Entry vector for Gateway with Eps8L2_297-370

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_297-370 (EPS8L2 Human)

Expression

Bacterial

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pROS_phleo-Tps1/Gsy2

Plasmid

#196612

Purpose

Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

TPS1, GSY2 (TPS1, GSY2 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G

Plasmid

#194462

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G

Plasmid

#194460

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-ADF_E

Plasmid

#146206

Purpose

Insect Expression of DmTral-ADF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-ADF (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-FAF_E

Plasmid

#146207

Purpose

Insect Expression of DmTral-FAF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FAF (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-FDA_E

Plasmid

#146208

Purpose

Insect Expression of DmTral-FDA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FDA (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pROS13-Tps2/Gsy1

Plasmid

#196613

Purpose

Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

S000001911 (TPS2, GSY1 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMeCP2Y pc644

Plasmid

#167565

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Insert

Methyl-CpG binding protein 2 (Mecp2 Rat)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2

Plasmid

#196534

Purpose

Expression of GST-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2_529-715

Plasmid

#196543

Purpose

Expression of myc-Eps8L2_529-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Tags

myc

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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GA46

Plasmid

#196614

Purpose

Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUGP1.1

Plasmid

#196615

Purpose

Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pv9_MBD1_MBD-R22A-13XL-BASU

Plasmid

#179417

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1.MBD-R22A-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dTLN1 exon

Plasmid

#176033

Purpose

A vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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