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C455-E01: attB4-stuffer-attB5

Plasmid

#162983

Purpose

Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C515-E01: attB5r-stuffer-attB1r

Plasmid

#162968

Purpose

Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP3

Plasmid

#65451

Purpose

pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Tags

yEGFP reporter

Expression

Yeast

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pITGFP3

Plasmid

#65452

Purpose

pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Tags

yEGFP-CLN2PEST reporter

Expression

Yeast

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
AAV-CAG-DIO-mAPX

Plasmid

#79908

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

mAPX

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Drosha3

Plasmid

#14776

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (Drosha Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pL59-Nat

Plasmid

#140465

Purpose

CRISPR/Cas9 engineering with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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miniSOG-Display-5

Plasmid

#54822

Purpose

Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

miniSOG-Display

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBS NL4-3 envFS eGFP Δgag/pol

Plasmid

#101343

Purpose

Deletion from the start of gag until 229 bases before the cPPT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta GagPol

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mEYFP

Plasmid

#87666

Purpose

retroviral vector encoding cre dependent expression of membrane EYFP

Depositor

Lynette Lim, Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-EYFP

Use

Retroviral

Tags

palmitylation

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPU-Empty-miR30-L1221

Plasmid

#132940

Purpose

All-in-one control rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

GFP

Use

Lentiviral and RNAi

Tags

HA

Expression

Mammalian

Promoter

SFFV

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pKT-SCFP3A-HIS

Plasmid

#64686

Purpose

pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

SCFP3A

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD2 SV40-AcrIIA4 (GB3333)

Plasmid

#160605

Purpose

SV40-AcrIIA4 codon optimized for N.benthamiana

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

Insert

AcrIIA4

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHR'-ROMK1-CITE-GFP

Plasmid

#21322

Depositor

Jay Nadeau

Article

Nadeau et al J Neurophysiol. 2000 Aug . 84(2):1062-75

Insert

ROMK1 (Kcnj1 Rat)

Use

Lentiviral

Tags

GFP (bicistronic with CITE/IRES)

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
LC3-mEYFP

Plasmid

#182865

Purpose

Autophagy Marker

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

rat LC3 (Anxa3 Rat)

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-MKRN1 H307E

Plasmid

#78749

Purpose

To overexpress MKRN1 H307E in Mammalian Cell

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

HA

Expression

Mammalian

Mutation

H307E

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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ptreCas9-mKate2ps-T1gRNA

Plasmid

#62715

Purpose

Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-BFLF1

Plasmid

#162661

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSR9.1347

Plasmid

#39540

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
VV221: sdChR(C138S)-TS-eGFP-ER in fck

Plasmid

#58513

Purpose

Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S)-TS-eGFP-ER

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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