We narrowed to 28,304 results for: cmv promoter

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pVimentin-miRFP670nano

Plasmid

#127436

Purpose

Vimentin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

Vimentin-miRFP670nano

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS7/myc-His

Plasmid

#127488

Purpose

Expresses myc-tagged NDUFS7 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS7 (NDUFS7 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-LucWT-GFP

Plasmid

#127709

Purpose

The expressed ORF encodes nucleus location signal with GFP and WT Luciferase.

Depositor

Xiaolu Yang

Article

Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

Insert

Luciferase

Tags

EGFP and FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-LucDM-GFP

Plasmid

#127796

Purpose

The expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.

Depositor

Xiaolu Yang

Article

Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

Insert

destabilized mutant (DM) Luciferase

Tags

EGFP and FLAG-NLS

Expression

Mammalian

Mutation

R188Q, R261Q in Firefly Luciferase insert

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-DAP5-3XFLAG

Plasmid

#127330

Purpose

Express 3xFLAG tagged DAP5 protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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AsCas12a-2C-NLS in pCSDest

Plasmid

#126637

Purpose

Expresses AsCas12a-2C-NLS in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

Plasmid

#127312

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-Gqi9

Plasmid

#125711

Purpose

Eukaryotic expression of the chimeric G protein Gqi9

Depositor

Kai Johnsson

Article

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Insert

chimericG protein Gqi9

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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A3Bi-Cas9n-UGI

Plasmid

#119135

Purpose

Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3B L1 intron-Cas9 nickase-UGI

Use

CRISPR

Mutation

L1 intron insertion

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP L138 Reporter

Plasmid

#119130

Purpose

Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

mCherry and eGFP

Use

Lentiviral

Mutation

eGFP L138S

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI

Plasmid

#126480

Purpose

Expresses Cas9-GSAlinker-TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

Cas9
GSA linker
TdT (DNTT Human)

Expression

Mammalian

Mutation

Changed codons to reduce repetitive sequences.

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS

Plasmid

#124705

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pQ2aB

Plasmid

#124887

Purpose

Basic Retroviral backbone with multiple 2a sites, linkers, BFP and and IRES_puromycin from pQCXIP

Depositor

Nevil Singh

Type

Empty backbone

Use

Retroviral

Tags

Blue Flourescent Protein, Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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HA-hNPHP1

Plasmid

#122872

Purpose

Expresses HA-tagged human NPHP1 in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Hoque et al Reproduction. 2021 Jul 8;162(2):129-139.

Insert

human NPHP1 (NPHP1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-Clover-Prx2-mRuby2

Plasmid

#117907

Purpose

fluorescent reporter of cytosolic hydrogen peroxide

Depositor

Hadley Sikes

Article

Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

Insert

Clover-Prx2-mRuby2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX301-KANK1

Plasmid

#121985

Purpose

overexpress of KANK1

Depositor

GuangJun Zhang

Article

Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

Insert

KANK1 (KANK1 Human)

Use

Lentiviral

Promoter

CMV promoter

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-PRM-5R

Plasmid

#112155

Purpose

Mammalian expression plasmid for PRM-5R in a vector expressing eGFP.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-5R (ABL1 Human)

Expression

Mammalian

Mutation

contains only the PRM domain of Abl1

Promoter

CMV

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Olig1 [N149/25.1R]

Plasmid

#114540

Purpose

Mammalian Expression Plasmid of anti-Olig1 (Mouse). Derived from hybridoma N149/25.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Olig1 (Mus musculus) recombinant mouse monoclonal antibody (Olig1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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FRB_nSbMVp

Plasmid

#118969

Purpose

Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nSbMVp in fusion with FRB

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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AP4mS_PPVs_P3_cLuc

Plasmid

#119300

Purpose

Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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