We narrowed to 42,866 results for: Tro
-
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Tt
Plasmid#154124PurposeExpresses wt Ribo-T rRNA and a set of tRNAs in bacterial cellsDepositorInsertsengineered bacterial rRNAs operon under control of pL promotor
bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
UseSynthetic BiologyExpressionBacterialPromoterPtac and pLAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRFP shRNA-resistant TrkB WT
Plasmid#72714PurposeMammalian expression of TrkB fused to RFP in C-terminus, shRNA resistantDepositorInsertTrkB
TagsRFPExpressionMammalianMutationshRNA-resistant TrkB (T300C, T301A, C302G, C306A,…PromoterCMVAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-004
Plasmid#78691PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-003
Plasmid#78690PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 WT
Plasmid#66139PurposeExpresses the wild type of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase0
Plasmid#59122PurposeFAK fret control sensor without FERM domain with no linkerDepositorInsertFocal Adhesion Kinase
TagsFLAG, mCerulean, and mCitrineExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AEB-DNT3
Plasmid#63852PurposeThis pFTV1 vector containsa 2,4,dinitrotoluene (DNT) binding riboswitch, controlling the translation initaition rate of mRFP1 gene.DepositorInsertDNT3 DNT riboswitch
UseSynthetic BiologyPromoterAEB3Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-N
Plasmid#113754PurposeGateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA2
Plasmid#73982PurposeOtx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.DepositorInsertEGFP-Otx2-shRNA2
UseRNAiExpressionMammalianPromoterCAGAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKT-mCitrineV163A-HIS
Plasmid#64685PurposepKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.DepositorInsertmCitrine V163A
ExpressionYeastMutationValine 163 changed to alanineAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1 Sesn1b
Plasmid#61874Purposebacterial expression of a GST-Sesn1b (mouse)DepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFPmAID_htz-1
Plasmid#174214PurposeDonor plasmid for tagging htz-1 with GFP::mAIDDepositorInsertGFP::mAID::htz-1 tagging donor
UseCRISPRExpressionWormAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TC393
Plasmid#122625PurposePser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertrtetR-QFAD::P2A::mKate::T2A::tetR-pie1
ExpressionWormAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-CrxshRNA
Plasmid#73987PurposeCrx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Crx gene
ExpressionMammalianPromoterCAGAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKC123
Plasmid#134240Purposeexpresses gRNA targeting CENP A 3'DepositorInsertCENP A 3' CRISPR construct
UseCRISPRExpressionMammalianAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top-Toehold-mCherry
Plasmid#87318PurposeExpresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.DepositorInsertSplit-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA1
Plasmid#73981PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse Otx2 gene
ExpressionMammalianPromoterCAGAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRISPRmTmG2
Plasmid#69992PurposeThe CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.DepositorInsertgRNA that targets near LoxP sites
Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only