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pnEK-NvSHN-DmeIF4E_Z

Plasmid

#148198

Purpose

Bacterial Expression of DmeIF4E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4E

Expression

Bacterial

Mutation

3 silent mutations compared to the sequence given…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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SpAGS-dGoLoco1-GFP

Plasmid

#185577

Purpose

for overexpression

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-dGoLoco1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

deletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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LDB73

Plasmid

#185437

Purpose

NUP1 CEN URA3 vector for synthetic lethal sectoring assays and screening

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

NUP1

Expression

Yeast

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-matDYK

Plasmid

#115258

Purpose

For mammalian expression of the human NODAL open reading frame with an internal DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS

Plasmid

#115250

Purpose

For in vitro transcription of human NODAL open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMA3998rc

Plasmid

#184851

Purpose

A retroviral vector with blasticidin resistance for cloning inserts between loxP sites

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-myc

Plasmid

#185495

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Yan et al Dev Biol. 2009 May 1;329(1):80-95. doi:

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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Sp(AU)sgRNA-mKO2

Plasmid

#184443

Purpose

sgRNA with AU flip (sgRNA2.0), mKO2 as selection marker

Depositor

Rhys Allan

Article

Chan et al Immunol Cell Biol. 2020 Sep 24. doi: 10.

Insert

sgRNA with AU flip, mKO2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-LgBiT-GAI

Plasmid

#183405

Purpose

Expresses nuclear-localized LgBiT-GAI

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

LgBiT-GAI

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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K0NHF1

Plasmid

#163203

Purpose

Inducible expression of UniProt identifier K0NHF1

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

K0NHF1

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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R0EVG9

Plasmid

#163290

Purpose

Inducible expression of UniProt identifier R0EVG9

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

R0EVG9

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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F4G5A4

Plasmid

#163219

Purpose

Inducible expression of UniProt identifier F4G5A4

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

F4G5A4

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Q15TJ7

Plasmid

#163285

Purpose

Inducible expression of UniProt identifier Q15TJ7

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

Q15TJ7

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0T9WB25

Plasmid

#163264

Purpose

Inducible expression of UniProt identifier A0A0T9WB25

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0T9WB25

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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A9D099

Plasmid

#163206

Purpose

Inducible expression of UniProt identifier A9D099

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A9D099

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_28_0570_WT

Plasmid

#171642

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 28 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.28.0570

Tags

Myc-His

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_31_2000_WT

Plasmid

#171643

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 31 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.31.2000

Tags

Myc-His

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huMlCas12a

Plasmid

#174678

Purpose

Expresses 6xHis-MBP tagged MlCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huMlCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huBfCas12a

Plasmid

#174672

Purpose

Expresses 6xHis-MBP tagged BfCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huBfCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PKM-Halo

Plasmid

#175321

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PKM (PKM Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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