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pCM5.51

Plasmid

#19382

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

rme-2 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCM5.42

Plasmid

#19390

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

fog-2 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pGGEV_2'_XcmI-LacZ

Plasmid

#49303

Purpose

Golden Gate entry vector at position 2, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_2'_Linker

Plasmid

#49288

Purpose

Golden Gate entry vector at position 2, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-DAAO

Plasmid

#60344

Purpose

Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

Depositor

Vsevolod Belousov

Article

Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

Insert

DAAO

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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ATMY3

Bacterial Strain

#218771

Purpose

E. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::inact lambda::tolC

Depositor

Abhishek Chatterjee

Article

Italia et al Cell Chem Biol. 2018 Oct 18;25(10):1304-

Bacterial Resistance

Spectinomycin

Species

Escherichia coli

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
ATMY4

Bacterial Strain

#218772

Purpose

E. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::CUA lambda::tolC

Depositor

Abhishek Chatterjee

Article

Italia et al Cell Chem Biol. 2018 Oct 18;25(10):1304-

Bacterial Resistance

Spectinomycin

Species

Escherichia coli

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
ATMY5

Bacterial Strain

#218773

Purpose

E. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS tyrTV::inact tyrU::inact lambda::tolC

Depositor

Abhishek Chatterjee

Article

Italia et al Cell Chem Biol. 2018 Oct 18;25(10):1304-

Bacterial Resistance

Spectinomycin

Species

Escherichia coli

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

Plasmid

#192636

Purpose

Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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myc-GABAAR Gamma2

Plasmid

#127619

Purpose

N-terminal myc-tagged Gamma2 subunit of GABAAR

Depositor

Josef Kittler

Article

Arancibia-Carcamo et al Proc Natl Acad Sci U S A. 2009 Oct 13;10

Insert

GABA-A receptor subunit gamma-2

Tags

myc

Expression

Mammalian

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-VMAT2-WPRE-pA

Plasmid

#39339

Depositor

Bernardo Sabatini

Article

Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV; R

Mutation

Myc tag inserted in the first lumenal loop betwee…

Available Since

Oct. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-jGCaMP7b-XC

Plasmid

#182221

Purpose

Improved genetic encoded calcium sensor based on GCaMP7b.

Depositor

Xiaodong Liu

Article

Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XC

Use

AAV

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p3HA-hMIB2

Plasmid

#33312

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB2 (MIB2 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
pDEST40-2XFL-Src-KD

Plasmid

#140313

Purpose

Expresses Flag-tagged human Src-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Contains only the KD region

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-recA56

Plasmid

#89962

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet and proD

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual α1B/β2B Tubulin

Plasmid

#170553

Purpose

Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Article

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB2B (TUBB2B Human)

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual α1B/β3 Tubulin

Plasmid

#170552

Purpose

Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Article

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB3 (TUBB3 Human)

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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