We narrowed to 1,469 results for: MiCy

1. pFBD-Akt13C(WT)

   Plasmid

   #86576

   Purpose

   Expresses cleavable human full-length Akt1(WT)

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-Gm129-Flag

   Plasmid

   #53766

   Purpose

   baculovirus expression of Gm129

   Depositor

   Aziz Sancar

   Article

   Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

   Insert

   Gm129 (Ciart Mouse)
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCas3cRh

   Plasmid

   #133773

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Joseph Bondy-Denomy

   Article

   Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   Cas7
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   polh, CAG, T7

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIB-GST-3C-CDK1

   Plasmid

   #177011

   Purpose

   Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CDK1 (CDK1 Human)
   

   Tags

   GST-3C

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac Dual α1B/β2B Tubulin

   Plasmid

   #170553

   Purpose

   Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

   Depositor

   Tarun Kapoor

   Article

   Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

   Inserts

   TUBA1B (TUBA1B Human)
   TUBB2B (TUBB2B Human)
   

   Tags

   AP linker, GS linker (GGSGG), His10 tag, L21 enha…

   Expression

   Insect

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac Dual α1B/β3 Tubulin

   Plasmid

   #170552

   Purpose

   Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

   Depositor

   Tarun Kapoor

   Article

   Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

   Inserts

   TUBA1B (TUBA1B Human)
   TUBB3 (TUBB3 Human)
   

   Tags

   AP linker, GS linker (GGSGG), His10 tag, L21 enha…

   Expression

   Insect

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcBac1.tR4-AcdRS82

   Plasmid

   #174101

   Purpose

   Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

   Depositor

   Ryan Mehl

   Article

   Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

   Insert

   M. barkeri tRNA synthetase for acridone82
   

   Expression

   Mammalian

   Mutation

   N311S, C313G, V366A, W382T

   Promoter

   CMV

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTA-Mob 2.0 Tp

   Plasmid

   #188619

   Purpose

   Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Conjugation helper plasmid

   Expression

   Bacterial and Yeast

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Jag1-HA pIRES

    Plasmid

    #17336

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Jagged1 (Jag1 Rat)
    

    Tags

    HA X3

    Expression

    Mammalian

    Mutation

    A silent C->G mutation at 3237

    Available Since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFBD-Akt13C(S473D)

    Plasmid

    #86577

    Purpose

    Expresses cleavable human full-length Akt1(S473D) phosphomimetic

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130; S473D phos…

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFl Strep-sumo-TEV-hAgo2

    Plasmid

    #136235

    Purpose

    Expresses human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Tags

    Strep Sumo

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcBac1-GFP(39TAG)

    Plasmid

    #217360

    Purpose

    Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    Enhanced green fluorescent protein
    

    Use

    Baculoviral

    Expression

    Insect and Mammalian

    Mutation

    Y39TAG

    Promoter

    CMV

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MultiMate 5 colors

    Plasmid

    #206266

    Purpose

    Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
    

    Use

    Recombinant baculovirus production, cre acceptor…

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN_TT 3xFLAG-KPNB1

    Plasmid

    #192301

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNB1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNB1 (KPNB1 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid

    #217896

    Purpose

    Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Tags

    2xSTII

    Expression

    Insect

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACEBac1-PSMA5-PSMA6-PSMA7

    Plasmid

    #217894

    Purpose

    Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMA5 (PSMA5 Human)
    PSMA6 (PSMA6 Human)
    PSMA7 (PSMA7 Human)
    

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN_TT 3xFLAG-KPNA2

    Plasmid

    #192306

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNA2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNA2 (KPNA2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-beta-mmr

    Plasmid

    #169241

    Purpose

    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    mutL-E36K
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed glutamic acid 36 to lysine

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACE-K2P10.1-StrepTagII

    Plasmid

    #191475

    Purpose

    Expresses K2P10.1 with C-terminal StrepTagII

    Depositor

    Arthur Laganowsky

    Article

    Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

    Insert

    K2P10.1 (KCNK10, TREK-2 Human)
    

    Expression

    Insect

    Mutation

    N144Q (uniprot P57789-1)

    Promoter

    polH

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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