Skip to main content
Addgene
Showing: 1161 - 1180 of 2007 results

  5. pAGH23

    Plasmid
    #107262
    Purpose
    contains CurT-mTurquoise2 construct used to replace the native CurT in Synechocystis PCC 6803 via homologous recombination.
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    curT (slr0483 )
    Use
    Synthetic Biology
    Tags
    mTurquoise2
    Expression
    Bacterial
    Mutation
    Promoter
    native CurT promoter
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107262-plasmid-map-sequence-id-207560

    Enlarge

  12. psBIG1a ActBT2ATetP2AP - H

    Plasmid
    #229972
    Purpose
    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Insert
    ActBT2ATetP2AP - H
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229972-plasmid-map-sequence-id-461256

    Enlarge

  13. psBIG1a ActBT2AmcherryP2AP -TagBFPH

    Plasmid
    #229971
    Purpose
    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Insert
    ActBT2AmcherryP2AP -TagBFPH
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229971-plasmid-map-sequence-id-461254

    Enlarge

  15. pSEVA63_HelperĪ”gIII

    Plasmid
    #190433
    Purpose
    M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.
    Depositor
    Manish Kushwaha
    Article
    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.
    Insert
    M13 genes I-V and VII-XI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    190433-plasmid-map-sequence-id-377863

    Enlarge

  16. pHBJT033

    Plasmid
    #225179
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (GmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225179-plasmid-map-sequence-id-444689

    Enlarge

  18. pCL1177

    Plasmid
    #217961
    Purpose
    Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3 (HIST2H3PS2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217961-plasmid-map-sequence-id-430215

    Enlarge

Showing: 1161 - 1180 of 2007 results