We narrowed to 1,537 results for: calcium

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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

Plasmid

#194683

Purpose

CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

Plasmid

#127392

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Aspartic acid and Threon…

Promoter

pCAG

Available Since

July 3, 2020

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

Plasmid

#218863

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8m

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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HBT-CPK32-Flag

Plasmid

#107432

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6-150

Plasmid

#91777

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-150

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

Plasmid

#194682

Purpose

CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

Plasmid

#194693

Purpose

CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Caprola_on-mEGFP

Plasmid

#194694

Purpose

CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

Plasmid

#194695

Purpose

CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

Plasmid

#194685

Purpose

CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_09-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

Plasmid

#111394

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
GCaMP6f

Use

AAV

Tags

6xHis, Myc, T7, and Xpress

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

Plasmid

#194686

Purpose

CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

Plasmid

#194687

Purpose

CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

Plasmid

#111393

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
GCaMP6f

Use

AAV

Tags

6xHis, Myc, T7, and Xpress

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

Plasmid

#194690

Purpose

hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

Plasmid

#194691

Purpose

hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-Cameleon

Plasmid

#123350

Purpose

Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-Cameleon

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6-210

Plasmid

#91778

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-210

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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