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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1161 - 1180 of 1485 results

  1. PDXK

    Plasmid
    #25535
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PDXK (PDXK Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pET32a-His-FADD

    Plasmid
    #78803
    Purpose
    To purify His-tagged recombinant FADD protein
    Depositor
    Jaewhan Song
    Insert
    GIG3 (FADD Human)
    Tags
    6x His, S-Tag, Thrombin cleavage site, and TrxA (…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pCarCBAE

    Plasmid
    #117755
    Purpose
    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Publication
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117755-plasmid-map-sequence-id-229025

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  15. Galt1-sCGrx1p-HA

    Plasmid
    #230028
    Purpose
    Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Galt1-sCGrx1p-HA (B4GALT1 Human, Synthetic)
    Tags
    HA
    Expression
    Mammalian
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230028-plasmid-map-sequence-id-458168

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  16. pSecUAG_UTu1D

    Plasmid
    #233867
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant
    Depositor
    Natalie Krahn, Dieter Söll
    Inserts
    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG) and …
    Promoter
    EM7, araBAD, and native As SelD
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233867-plasmid-map-sequence-id-471093

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  17. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  19. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid
    #227245
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell
    Depositor
    Jae Seong Lee
    Publication
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227245-plasmid-map-sequence-id-450568

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Showing: 1161 - 1180 of 1485 results