We narrowed to 11,822 results for: nts
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Plasmid#114074PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationS542R, K548V and N552RAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN
Plasmid#69584PurposeExpresses mutated p53-L344A tagged with mKate2 and split N-term mVenusDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pJT226 lenti pEF-rTetR(SE-G72P)-3XFLAG-SMARAC2 QLQ-T2A-mCherry-BSD-WPRE
Plasmid#188761PurposeLentiviral backbone containing SMARCA2 QLQ as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertSMARCA2 QLQ (SMARCA2 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-ADF_E
Plasmid#146206PurposeInsect Expression of DmTral-ADFDepositorInsertDmTral-ADF (tral Fly)
ExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-FAF_E
Plasmid#146207PurposeInsect Expression of DmTral-FAFDepositorInsertDmTral-FAF (tral Fly)
ExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-FDA_E
Plasmid#146208PurposeInsect Expression of DmTral-FDADepositorInsertDmTral-FDA (tral Fly)
ExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-BQ.1.1-AVI
Plasmid#196621PurposeExpresses SARS-CoV-2 S2P protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-BQ.1.1 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24-, P25-, P26-, A27S, H69-, V70-, G142D, …Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
Plasmid#172599PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.DepositorInsertmyc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842V+R914WPromoterCMVAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter
Plasmid#172600PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.DepositorInsertmyc-PDGFRA-K627R+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627R+R914WPromoterCMVAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO2-RC_B
Plasmid#145981PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
ExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-DFDF-KFKF_C
Plasmid#146038PurposeInsect Expression of DmTral-DFDF-KFKFDepositorInsertDmTral-DFDF-KFKF (tral Fly)
ExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Noxa)-pEGFP-C1
Plasmid#166761PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of NoxaDepositorTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Noxa…PromoterCMVAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Bik)-pEGFP-C1
Plasmid#166760PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BikDepositorTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Bik …PromoterCMVAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pVRC8400-SARS-CoV-2-S2P-BJ.1-AVI
Plasmid#196620PurposeExpresses SARS-CoV-2 S2P protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-BJ.1 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-dsRed-attP_dCrk_flankingHRs
Plasmid#131141PurposeContains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).DepositorUseCRISPR and Cre/Lox; Homology directed repair temp…Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
KHBD00450
Plasmid#39563DepositorAvailable SinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
KHBD00026
Plasmid#34188DepositorInsertCG8333 (E(spl)mgamma-HLH Fly)
UseGateway CloningAvailable SinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C
Plasmid#146039PurposeInsect Expression of DmTral-FDF-ADADepositorInsertDmTral-FDF-ADA (tral Fly)
ExpressionInsectMutationFDF to ADA (407-409) mutation. Additionally, thre…Available SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-bio-myc-cCE
Plasmid#112711PurposeFor tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
TagsHIV Rev nuclear localization signal (NES), Stop c…ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMV with Tet repressor binding sitesAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only