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pMOD_B4800EC

Plasmid

#176874

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2

Plasmid

#176864

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG3

Plasmid

#176866

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_3

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4

Plasmid

#176867

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E

Plasmid

#146241

Purpose

Insect Expression of DmSMG6-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

DmSMG6-siRNAres (Smg6 Fly)

Expression

Insect

Mutation

T151A, deleted A947

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0180

Plasmid

#172814

Purpose

Level 1 module containing TP-CFP_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-CFP-3U+TER35S

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0140

Plasmid

#172794

Purpose

Level 0 module containing CDS2ns_mCHERRY

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mCHERRY

Use

Synthetic Biology

Mutation

no

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0081

Plasmid

#172787

Purpose

Level 0 module containing CT_nVenus

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

3xFLAG CtmVenus 210-238 STOP pAGM1301 (CT)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0090

Plasmid

#172788

Purpose

Level 0 module containing NT2_Start

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer module with start codon.

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0110

Plasmid

#172790

Purpose

Level 0 module containing CDS1_spacer

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Empty spacer module

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0130

Plasmid

#172792

Purpose

Level 0 module containing CDS2ns_mPTAC5

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature PTAC5 (41-387), Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0071

Plasmid

#172785

Purpose

Level 0 module containing CT_nVenus

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer+NtVenus 1-210 STOP pAGM1301 (CT)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Cdc42

Plasmid

#180582

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_57-499_B

Plasmid

#145978

Purpose

Insect Expression of DmHPat_57-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-499 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_1-56_A

Plasmid

#145869

Purpose

Insect Expression of DmHPat_1-56

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_1-56 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_1-499_A

Plasmid

#145871

Purpose

Insect Expression of DmHPat_1-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_1-499 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_226-499_A

Plasmid

#145872

Purpose

Insect Expression of DmHPat_226-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_226-499 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_226-499_A

Plasmid

#145877

Purpose

Insect Expression of DmHPat_226-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_226-499 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_500-968_A

Plasmid

#145878

Purpose

Insect Expression of DmHPat_500-968

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_500-968 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA

Plasmid

#170767

Purpose

Methylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Inserts

M.XbaI
M.EcoRI
M.SalI

Expression

Bacterial

Promoter

rhamnose

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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