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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,266 results for: crispr grnas

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Showing: 11801 - 11820 of 14266 results

  1. BE3-hA3A-R128A

    Plasmid
    #132944
    Purpose
    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-hA3A(R128A)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    BE3-hA3A(R128A)
    Available Since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132944-plasmid-map-sequence-id-314824

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  2. pSH-Csy4-T2A-SaRFN

    Plasmid
    #85755
    Purpose
    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-SadCas9
    Use
    CRISPR
    Tags
    3xHA tag, Csy4, and FokI fusion via 36 amino acid…
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    CAG
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85755-plasmid-map-sequence-id-170274

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  5. BE3-YE1

    Plasmid
    #132943
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-(W90Y-R126E)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    BE3-(W90Y-R126E)
    Available Since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132943-plasmid-map-sequence-id-314816

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  11. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Available Since
    Oct. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159752-plasmid-map-sequence-id-313824

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  15. pX459-puro-hBRAF-1

    Plasmid
    #185365
    Purpose
    For mammalian expression of guide RNA: caccgACAACAGTTATTGGAATCTC that targets human BRAF
    Depositor
    Ayaz Najafov
    Insert
    BRAF (BRAF Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185365-plasmid-map-sequence-id-363594

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  16. pX459-puro-hBRAF-2

    Plasmid
    #185366
    Purpose
    For mammalian expression of guide RNA: caccgAAAATCCCACAGATGTGGCA that targets human BRAF
    Depositor
    Ayaz Najafov
    Insert
    BRAF (BRAF Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185366-plasmid-map-sequence-id-363457

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  17. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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  18. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118163
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118163-plasmid-map-sequence-id-240400

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  19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118164
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118164-plasmid-map-sequence-id-240433

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  20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118161
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118161-plasmid-map-sequence-id-240357

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Showing: 11801 - 11820 of 14266 results