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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-H2BC11_sgRNA

Plasmid

#183883

Purpose

pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-MYH10_sgRNA

Plasmid

#183887

Purpose

pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-H2BC11_sgRNA

Plasmid

#183881

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-MYH10_sgRNA

Plasmid

#183886

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z

Plasmid

#148160

Purpose

Insect Expression of Dm4E-T_1-511-LLFWKtoAAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)

Expression

Insect

Mutation

alias: 4E-T; one non silent mutation L127S, annot…

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK91 pHomL-TDH3p-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179075

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)-Link-SCS2tmh

Expression

Yeast

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

Plasmid

#178986

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK66 pHomL-RPL18Bp-TOM70-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179050

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-TOM70-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK65 pHomL-RPL18Bp-preCOX4-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179049

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-preCOX4-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK64 pHomL-RPL18Bp-PEX25-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179048

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Neon(gfp), a marker for the peroxisome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PEX25-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK63 pHomL-RPL18Bp-PDR16-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179047

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PDR16-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK62 pHomL-RPL18Bp-ERG6-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179046

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ERG6-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK61 pHomL-RPL18Bp-Neon(gfp)-Link-ATG8-SSA1t-HomR (AmpR)

Plasmid

#179045

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-ATG8, a marker for the autophagosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-ATG8

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK60 pHomL-RPL18Bp-PRC1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179044

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Neon(gfp), a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PRC1-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK59 pHomL-RPL18Bp-Neon(gfp)-Link-VAM3-SSA1t-HomR (AmpR)

Plasmid

#179043

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VAM3, a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-VAM3

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK58 pHomL-RPL18Bp-Neon(gfp)-Link-YPT7-SSA1t-HomR (AmpR)

Plasmid

#179042

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-YPT7, a marker for the vacuole, late endosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-YPT7

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK57 pHomL-RPL18Bp-SNF7-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179041

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Neon(gfp), a marker for the endosome, class E compartment

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SNF7-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK56 pHomL-RPL18Bp-Neon(gfp)-Link-VPS21-SSA1t-HomR (AmpR)

Plasmid

#179040

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VPS21, a marker for the endosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-VPS21

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK55 pHomL-RPL18Bp-PIL1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179039

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Neon(gfp), a marker for the eisosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PIL1-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge