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pME-memGFP-P2A

Plasmid

#80809

Purpose

Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

pME-memGFP-P2A

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32474

Purpose

Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G-5HT3 HC

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pMUT2-VtrA-mCherry

Plasmid

#192862

Purpose

pMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

VtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

Plasmid

#183741

Purpose

Expresses Ki67 (8 repeats+LR)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

MKI67 LR domain + 8 Ki-67 repeats

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-N-Flag-8His

Plasmid

#157677

Purpose

N-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Flag-8His

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGADT7-αC

Plasmid

#197650

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-2 αC

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Ser677T substitution

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xhfYFP

Plasmid

#186544

Purpose

Hyperfolder YFP, triple fusion: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP (3X)

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKEK3395

Plasmid

#234079

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

YukonOFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Plasmid

#198450

Purpose

Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2A

Plasmid

#183324

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2A

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ChR2-mRuby2-ST

Plasmid

#109125

Purpose

Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChR2-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC-T7-Nla-repeat-BbsI

Plasmid

#180215

Purpose

For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec16B

Plasmid

#66607

Purpose

Expresses Sec16B as a GFP fusion

Depositor

David Stephens

Article

Budnik et al Sci Rep. 2011;1:77. doi: 10.1038/srep000

Insert

Sec16B (SEC16B Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSC10

Plasmid

#104784

Purpose

pAH595-gRNA 2xplex entry cassette

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK1006 (pSL5)

Plasmid

#191001

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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Avr2-GFP pZK538

Plasmid

#118011

Purpose

expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .

Depositor

FLW Takken

Article

et al Bio-protocol 8(24): e3114.

Inserts

dspAvr2
mCherry

Tags

EGFP and HDEL

Expression

Bacterial and Plant

Promoter

35S

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-CAG::ChR2-YFP

Plasmid

#59740

Purpose

Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Channelrhodopsin-2

Tags

YFP

Expression

Mammalian

Mutation

H134R

Promoter

CAG

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
[pSK104] pET28 SLC38A9N-His

Plasmid

#136142

Purpose

bacterial expression of SLC38A9 N term fragment (1-119)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

SLC38A9 N term fragment (1-119) (SLC38A9 Human)

Tags

His

Expression

Bacterial

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-4CL1

Plasmid

#35947

Depositor

Claudia Schmidt-dannert

Article

Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

Insert

4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLac-mod

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only

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LAMP1-ABKAR

Plasmid

#65068

Purpose

Expression of ABKAR biosensor targeted to the lysozome

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

ABKAR biosensor

Tags

Lamp1

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only