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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 96,342 results for: nin

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Showing: 11941 - 11960 of 96342 results

  2. R777-E135 Hs.MRAS

    Plasmid
    #70419
    Purpose
    Gateway ORF clone of human MRAS [NM_001085049.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MRAS (MRAS Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70419-plasmid-map-sequence-id-125868

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  3. pFHMSP-LIC-C

    Plasmid
    #26100
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    8x His tag and MKFLVNVALVFMVVYISYIYAAA (Honeybee …
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  4. opto-Rac1

    Plasmid
    #135396
    Purpose
    Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Publication
    Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    opto-Rac1
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135396-plasmid-map-sequence-id-271132

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  5. pET-EIN-AlkBH5(66-292)

    Plasmid
    #125732
    Purpose
    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.
    Depositor
    Vincenzo Venditti
    Publication
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    AlkBH5(66-292) (ALKBH5 Human)
    Tags
    6xHis and EIN
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125732-plasmid-map-sequence-id-244619

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  7. pTre-Tight-luciferase-GFP (467)

    Plasmid
    #65491
    Purpose
    negative control for expression assays
    Depositor
    Roderic Guigo, Rory Johnson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65491-plasmid-map-sequence-id-148973

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  9. pCMV/MASC_(GLuc)_TOP1

    Plasmid
    #68439
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68439-plasmid-map-sequence-id-120217

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  13. TBG-Vector

    Plasmid
    #109396
    Purpose
    AAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liver
    Depositor
    Sidi Chen
    Publication
    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:
    Type
    Empty backbone
    Use
    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…
    Expression
    Mammalian
    Promoter
    TBG
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109396-plasmid-map-sequence-id-222776

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  14. pDHt/sk-PC

    Plasmid
    #92125
    Purpose
    Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Publication
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR; Trichoderma reesei expression
    Promoter
    Ppdc
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92125-plasmid-map-sequence-id-215731

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  20. mKate2-P2A-APEX2-OSBP

    Plasmid
    #67664
    Purpose
    Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Publication
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mKate2-P2A-APEX2-OSBP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67664-plasmid-map-sequence-id-117189

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Showing: 11941 - 11960 of 96342 results