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Viral Prep#213940-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiPVe3_dTomato_nlsdTomato (#213940). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the PV+ basket cell-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAAV_BiPVe4_dTomato_nlsdTomato (AAV PHP.eB)
Viral Prep#213936-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiPVe4_dTomato_nlsdTomato (#213936). In addition to the viral particles, you will also receive purified pAAV_BiPVe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the chandelier cell-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiCHATe27_dTomato_nlsdTomato (AAV PHP.eB)
Viral Prep#213829-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_dTomato_nlsdTomato (#213829). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiLAMP5e3_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213915-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiLAMP5e3_ChR2_mCherry (#213915). In addition to the viral particles, you will also receive purified pAAV_BiLAMP5e3_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the Lamp5 interneuron-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe10_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213815-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiSSTe10_ChR2_mCherry (#213815). In addition to the viral particles, you will also receive purified pAAV_BiSSTe10_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the SST interneuron-targeting enhancer E10. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiVIPe4_dTomato_nlsdTomato (AAV PHP.eB)
Viral Prep#213855-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiVIPe4_dTomato_nlsdTomato (#213855). In addition to the viral particles, you will also receive purified pAAV_BiVIPe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the VIP interneuron-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiCHATe27_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213830-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_ChR2_mCherry (#213830). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213937-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiPVe4_ChR2_mCherry (#213937). In addition to the viral particles, you will also receive purified pAAV_BiPVe4_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the chandelier cell-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiVIPe4_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213856-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiVIPe4_ChR2_mCherry (#213856). In addition to the viral particles, you will also receive purified pAAV_BiVIPe4_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the VIP interneuron-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
puc19-pCas13a
Plasmid#118963PurposeIntermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.DepositorInsertCas13a
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_NbmiR164e/h
Plasmid#231157PurposeT-DNA encoding TRV2 with mobile gRNA targeting NbmiR164e/hDepositorInsertmobile gRNA targeting NbmiR164e/h
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_NbATML1-1pro
Plasmid#231151PurposeT-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1proDepositorInsertmobile gRNA targeting NbATML1-1pro
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_NbPDS
Plasmid#231147PurposeT-DNA encoding TRV2 with mobile gRNA targeting NbPDSDepositorInsertmobile gRNA targeting NbPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_SlMIR164b
Plasmid#231153PurposeT-DNA encoding TRV2 with mobile gRNA targeting SlMIR164bDepositorInsertmobile gRNA targeting SlMIR164b
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_SlMIR164b
Plasmid#231152PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting SlMIR164bDepositorInsertTREX2 and mobile gRNA targeting SlMIR164b
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_NbSTM-5'UTR
Plasmid#231150PurposeT-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTRDepositorInsertmobile gRNA targeting NbSTM-5?UTR
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_NbPDS
Plasmid#231146PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDSDepositorInsertTREX2 and mobile gRNA targeting NbPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-2_NbPDS
Plasmid#231145PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDSDepositorInsertTREX2 and mobile gRNA targeting NbPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_NbATML1-2pro and NbATML1-1pro
Plasmid#231155PurposeT-DNA encoding TRV2 with mobile gRNAs targeting NbATML1-2pro and NbATML1-1proDepositorInsertmobile gRNAs targeting NbATML1-2pro and NbATML1-1pro
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pABCb10-GFP
Plasmid#19031DepositorInsertATP-binding cassette, sub-family B (MDR/TAP), member 10 (Abcb10 Mouse)
UseTagsHISExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_NbmiR164e/h
Plasmid#231156PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbmiR164e/hDepositorInsertTREX2 and mobile gRNA targeting NbmiR164e/h
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_NbATML1-1pro
Plasmid#231149PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbATML1-1proDepositorInsertTREX2 and mobile gRNA targeting NbATML1-1pro
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_NbSTM-5'UTR
Plasmid#231148PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbSTM-5?UTRDepositorInsertTREX2 and mobile gRNA targeting NbSTM-5?UTR
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
EC1000
Bacterial Strain#71852PurposeRepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmidsDepositorBacterial ResistanceKanamycinAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDL639
Plasmid#231163PurposeT-DNA encoding TRV2 with mobile gRNAs targeting SlHAIRPLUS and SlAN2DepositorInsertmobile gRNAs targeting SlHAIRPLUS and SlAN2
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDL649
Plasmid#231164PurposeT-DNA encoding TRV2 with mobile gRNA targeting SlHAIRLESSDepositorInsertmobile gRNA targeting SlHAIRLESS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDL518
Plasmid#231160PurposeT-DNA encoding TRV2 with mobile gRNA targeting SlPDSDepositorInsertmobile gRNA targeting SlPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEE771
Plasmid#231159PurposeT-DNA encoding TRV2 with mobile gRNA targeting SlPDSDepositorInsertmobile gRNA targeting SlPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEE485
Plasmid#231158PurposeT-DNA encoding TRV2 with mobile gRNA targeting SlPDSDepositorInsertmobile gRNA targeting SlPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_NbATML1-2pro and NbATML1-1pro
Plasmid#231154PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNAs targeting NbATML1-2pro and NbATML1-1proDepositorInsertTREX2 and mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDL562
Plasmid#231162PurposeT-DNA encoding TRV2 with mobile gRNA targeting SlCHL1DepositorInsertmobile gRNA targeting SlCHL1
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEE834
Plasmid#231168PurposeT-DNA encoding TRV2 with mobile gRNA targeting SlPDSDepositorInsertmobile gRNA targeting SlPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
IF189
Bacterial Strain#134836PurposeRapid and efficient recombineering and purificiation of modified lambda phage DNADepositorBacterial ResistanceNoneAvailable sinceJan. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
OpLac2
Bacterial Strain#52699PurposeKIlac with a codon optimized lacZ using codons for a subset of amino acids read by transfer RNAs that are the most highly charged during amino acid starvationDepositorBacterial ResistanceNoneAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
S4197
Bacterial Strain#200839Purposewildtype strain MG1655 rph+, ilvG+, ΔlacZDepositorBacterial ResistanceNoneSpeciesEscherichia coli str. k-12 substr. mg1655Available sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Strain HK022 JQ929581
Bacterial Strain#38204DepositorBacterial ResistanceKanamycinSpeciesSyntheticAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Strain Phi80 JQ929581
Bacterial Strain#38202DepositorBacterial ResistanceKanamycinSpeciesSyntheticAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Strain Phi80 JQ929582
Bacterial Strain#38203DepositorBacterial ResistanceKanamycinSpeciesSyntheticAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK605
Plasmid#72530PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Z956
Bacterial Strain#200838PurposeHost strain for plasmids pYG205 and pYG215 or derivatives of the latterDepositorBacterial ResistanceNoneSpeciesEscherichia coli str. k-12 substr. mg1655Available sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK606
Plasmid#72529PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subunit with C-terminal His6 tag (AaPurLH6)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK618
Plasmid#72535PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His6 tag; glutaminase subunit; large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK619
Plasmid#72536PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3
Plasmid#48811PurposeThis report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.DepositorInsertnAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsExpressionMammalianMutation*PromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-YFP
Plasmid#50484Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsYFPExpressionMammalianMutationYFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-CFP
Plasmid#50485Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsCFPExpressionMammalianMutationCFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCB6-EnvA
Plasmid#74420PurposeMammalian expression of the subgroup A avian leukosis-sarcoma envelope glycoprotein (EnvA)DepositorInsertEnv from ASLV-A
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK625
Plasmid#72541PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)DepositorInsertshypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK040 (pET-PurQ-A128T)
Plasmid#73840PurposeProduces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationA128T mutantPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only