Addgene

We narrowed to 3,030 results for: AER

Showing: 101 - 120 of 3030 results

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pTAMAHISTEV_PfeAQ482A

Plasmid

#128945

Purpose

expresses Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Use

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP11 CS puroR

Plasmid

#46748

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT H335G

Plasmid

#13749

Depositor

Luca Pellegrini

Article

Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic His 335 to Gly

Promoter

Available since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
Rescue Septin 11 mRNA

Plasmid

#122025

Purpose

Rescue mRNA for Septin 11 Knock Down with sh2 shRNA. Splice variant II (EU711415) with 5 silent mutations at SEPT11 sh2 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (CUG…

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pS238D1::sptse5-CT

Plasmid

#192959

Purpose

For expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studies

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT

Use

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAR480A

Plasmid

#128944

Purpose

expresses R480A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Use

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAMAHISTEV_PfeAG324V

Plasmid

#128947

Purpose

expresses G324V mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Use

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBADHISTEV_PfeEH258A

Plasmid

#129122

Purpose

expresses H258A mutant of PfeE in E.coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed, mutat…

Promoter

PBAD

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeES157A

Plasmid

#129121

Purpose

expresses S157A mutant of PfeE in E.coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed, mutat…

Promoter

PBAD

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKTop::tse5-CT (1169-1281)

Plasmid

#192955

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1281)

Use

Tags

Expression

Bacterial

Mutation

encodes for tse5 (1169-1281)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAR480A-Q482A

Plasmid

#128946

Purpose

expresses R480A Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Use

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::sptse5-CT (1169-1229)

Plasmid

#192948

Purpose

For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT (1169-1229)

Use

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1229)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::tse5-CT (1169-1300)

Plasmid

#192956

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1300)

Use

Tags

Expression

Bacterial

Mutation

encodes for tse5 (1169-1300)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pKTop::tse5-CT (1169-1317)

Plasmid

#192957

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1317) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1317)

Use

Tags

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::tse5-CT (1169-1269)

Plasmid

#192954

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1269) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1269)

Use

Tags

Expression

Bacterial

Mutation

encodes for tse5 (1169-1269)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pS238D1::sptse5-CT_phoA-lacZalpha

Plasmid

#192961

Purpose

Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT_phoA-lacZ_

Use

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317) fused to phoA-lacZal…

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::tse5-CT (1169-1229)

Plasmid

#192953

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1229) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1229)

Use

Tags

Expression

Bacterial

Mutation

encodes for tse5 (1169-1229)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::sptse5-CT (1169-1281)

Plasmid

#192950

Purpose

For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT (1169-1281)

Use

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1281)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::sptse5-CT (1169-1269)

Plasmid

#192949

Purpose

For membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT (1169-1269)

Use

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1269)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::sptse5-CT (1169-1300)

Plasmid

#192951

Purpose

For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT (1169-1300)

Use

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for sptse5 (1169-1300)

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge