Purpose
Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.
Insert
Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
Use
CRISPR and Synthetic Biology
Promoter
J23119 and lac promoter
Available Since
Oct. 6, 2022
Availability
Academic Institutions and Nonprofits only