We narrowed to 837 results for: Anti-CRISPR

1. AcrIIC1-mCherry chimera #10

   Plasmid

   #128113

   Purpose

   Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

   Depositor

   Dominik Niopek

   Article

   Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

   Insert

   AcrIIC1-mCherry chimera #10
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyCas9(WT)

   Plasmid

   #101044

   Purpose

   Express Streptococcus pyogenes Cas9

   Depositor

   Zhiwei Huang

   Article

   Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

   Insert

   SpyCas9
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

   Plasmid

   #136513

   Purpose

   Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

   Insert

   codon-optimized AcrIIA5
   

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-SpyCas9

   Plasmid

   #113033

   Purpose

   SpyCas9 expression in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SpyCas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

   Plasmid

   #136514

   Purpose

   Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

   Depositor

   Erik Sontheimer

   Article

   Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

   Insert

   Codon-optimized AcrIIA5
   

   Use

   Lentiviral

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPgiCas13b-gRNA-NT

   Plasmid

   #184835

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS433-pCSDest2-AcrIIC1Nme

   Plasmid

   #85679

   Purpose

   Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidis

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Type II-C anti-CRISPR AcrIIC1Nme
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPbuCas13b-gRNA-NT

   Plasmid

   #184568

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEJS436-pCSDest2-AcrIIC2Nme

   Plasmid

   #85712

   Purpose

   Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Type II-C anti-CRISPR AcrIIC2Nme
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS443-pCSDest2-AcrIIC3Nme

    Plasmid

    #85713

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC3Nme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS430-pCSDest2-AcrIIC1Boe

    Plasmid

    #85678

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC1Boe
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS427-pCSDest2-AcrE2

    Plasmid

    #85677

    Purpose

    Mammalian expression of anti-CRISPR protein from Type I-E

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type I-E anti-CRISPR
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-SpyCas9

    Plasmid

    #113034

    Purpose

    AAV vector for expression of SpyCas9 in mammalian cells

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SpyCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPgiCas13b

    Plasmid

    #184833

    Purpose

    Inducible expression of PgiCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Cas13b nuclease from Porphyromonas gingivalis
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPlacPbuCas13b-gRNA-NT

    Plasmid

    #184838

    Purpose

    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and lac promoter

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. dSpyCas9-p300-P2A-CASANOVA

    Plasmid

    #113129

    Purpose

    Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    dCas9-p300-P2A-CASANOVA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid

    #184840

    Purpose

    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and lac promoter

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPgiCas13b-gRNA-1

    Plasmid

    #184834

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge