-
Plasmid#51466PurposeNon-PIP3 binding control for PH-Btk-GFPDepositorInsertAKT (aa 1–164)
UseTagsEGFPExpressionMammalianMutationR25CPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC1-PIP-SHOW
Plasmid#42212PurposeGenetically encoded fluorescent indicator for simultaneous PIP3 and hydrogen peroxide detection.DepositorInsertPIP-SHOW
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfp(ASV)C
Plasmid#111617PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfpC
Plasmid#111616PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1-GFP
Plasmid#180420PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL-1
Plasmid#180422PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.DepositorInsertRed Chromprotein gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1-E2C
Plasmid#180421PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.DepositorInsertE2 Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBAD-yhjH
Plasmid#111613PurposeArabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjHDepositorInsertyhjH
UseTagsExpressionBacterialMutationA52VPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Txk-SH2
Plasmid#46526DepositorInsertTxk (Txk Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 138-258PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SH3BP5
Plasmid#23579DepositorInsertSH3BP5 (SH3BP5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEBG-II-I Y248,249,611F
Plasmid#22251DepositorInsertTFII-I (GTF2I Human)
UseTagsGSTExpressionMammalianMutationTyrosine 248, 249 and 611 mutated to PhenylalaninePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGTF2I.1.0-gDNA
Plasmid#132452PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertGTF2I (GTF2I Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-I Y248&249F
Plasmid#22189DepositorInsertTFII-I (GTF2I Human)
UseTagsGSTExpressionMammalianMutationChanged Tyrosines 248 and 249 to Phenylalanines.PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAC17V5a
Plasmid#197413PurposeIPTG-inducible two-plasmid system bearing CP25 > lacO-GFP, specR, RSF1010 replicon (1/2)DepositorInsertlacO-GFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC17V5b
Plasmid#197414PurposeIPTG-inducible two-plasmid system bearing CP25 > lacI, ampR, pTF-FC2 replicon (2/2)DepositorInsertlacI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC04
Plasmid#197400PurposeHoney bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and pBBR1 repliconDepositorInsertpBBR1 replicon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC06
Plasmid#197401PurposeHoney bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and RK2 repliconDepositorInsertRK2 replicon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only