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Plasmid#53730PurposePlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.DepositorInsertbacteriophage lambda CI protein
UseTagsExpressionBacterialMutationPromoterlac UV5Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.PAC
Plasmid#57828PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
UseTagsExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRPaCas9
Plasmid#111819PurposeExpresses Cas9 in T. brucei (2T1) cellsDepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_2paCRY2
Plasmid#129651Purpose2P-activatable CRY2DepositorInsert2paCRY2
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCAGAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9
Plasmid#78903PurposeExpresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertdCas9
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCk2_spacer
Plasmid#136072PurposeUse when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk1_spacer
Plasmid#136071PurposeUse when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACM4
Plasmid#49797PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC574
Plasmid#127975PurposeAMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInserthygB (hygromycin resistance marker)
UseFungal expressionTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACT2.2
Plasmid#11343DepositorTypeEmpty backboneUseTagsGal4 Activation DomainExpressionYeastMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC37
Plasmid#111618PurposeFluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosaDepositorInsertPpqsA-gfp(ASV)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACT2.2gtwy
Plasmid#11346DepositorInsertgateway cassette
UseTagsGal4 Activation DomainExpressionYeastMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
HypaCas9
Plasmid#138557PurposeExpresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSDDepositorInsertHypaCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN692A, M694A, Q695A, H698APromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACU
Plasmid#58373PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC1750
Plasmid#127984PurposeAMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYC.GFP1Amb
Plasmid#102792PurposeExpresses GFP with a premature stop codonDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterialMutationY39StopPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only