pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)
Plasmid
#160736
Purpose
Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.
Depositor
Insert
VchCAST proteins, crRNA, and donor DNA
Use
Tags
Expression
Bacterial
Mutation
Promoter
J23119
Available since
March 10, 2021
Availability
Academic Institutions and Nonprofits only
