We narrowed to 962 results for: cas12a

1. pYL-kasOp-FnCas12a2

   Plasmid

   #177874

   Purpose

   Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

   Depositor

   Yunzi Luo

   Article

   Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

   Insert

   Streptomyces codon optimized FnCas12a
   

   Use

   CRISPR

   Promoter

   kasOp*

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-dCas12a-ABE7

   Plasmid

   #193644

   Purpose

   Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA-TadA*7.10, EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-enCas12a-2xNLS

   Plasmid

   #209021

   Purpose

   Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   enCas12a-2xNLS-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   E174R/S542R/K548R

   Promoter

   EF-1a

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHis-MBP-huErCas12a

   Plasmid

   #174685

   Purpose

   Expresses 6xHis-MBP tagged ErCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huErCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis

   Expression

   Bacterial

   Mutation

   Q926R mutation is observed as indicated from the …

   Promoter

   T7

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-dCas12a-A3A

   Plasmid

   #193638

   Purpose

   Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A, EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti-Lb-Cas12a-2xNLS

   Plasmid

   #155046

   Purpose

   Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS, Myc-tag and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LbCas12a-2C-NLS in pCSDest

   Plasmid

   #126638

   Purpose

   Expresses LbCas12a-2C-NLS in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Promoter

   CMV IE94 promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQdCas12a.luxR(mut)-sggfp(C)

   Plasmid

   #236043

   Purpose

   The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Article

   Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
   

   Use

   CRISPR

   Promoter

   quorum sensing promoter

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQdCas12a.luxR(mut)-empty

   Plasmid

   #236037

   Purpose

   The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Article

   Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI, dcas12a, and lacZ-alpha (fragment).
   

   Use

   CRISPR

   Promoter

   quorum sensing promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ230-D156R (ttLbCas12a)

    Plasmid

    #195366

    Purpose

    A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    LbCas12a-D156R (ttLbCas12a)
    

    Use

    CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

    Expression

    Plant

    Promoter

    ZmUBI1

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

    Plasmid

    #176250

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-HkCas12a-2AeGFP

    Plasmid

    #123634

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    HkCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis-MBP-huBoCas12a

    Plasmid

    #174673

    Purpose

    Expresses 6xHis-MBP tagged BoCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBoCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 6xHis-MBP-huPb2Cas12a

    Plasmid

    #174686

    Purpose

    Expresses 6xHis-MBP tagged Pb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJCC_076 AsCas12a R1226A

    Plasmid

    #171669

    Purpose

    For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a R1226A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginine 1226 to alanine

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AsCas12a-2C-NLS in pCSDest

    Plasmid

    #126637

    Purpose

    Expresses AsCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Promoter

    CMV IE94 promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-AsCas12a-2AeGFP

    Plasmid

    #123638

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    AsCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBP-LbCas12a D832A

    Plasmid

    #113517

    Purpose

    Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    D832A

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-PxCas12a-2AeGFP

    Plasmid

    #123637

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PxCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. FnoCas12a-2C-NLS in pCSDest

    Plasmid

    #126639

    Purpose

    Expresses FnoCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Promoter

    CMV IE94 promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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