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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,112 results for: cas12a

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Showing: 101 - 120 of 1112 results

  1. pAsCpf1(TATV)(BB) (pY221)

    Plasmid
    #89354
    Purpose
    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1 RVR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    S542R/K548V/N552R
    Promoter
    CBh
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89354-plasmid-map-sequence-id-182593

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  2. pPB12A

    Plasmid
    #210035
    Purpose
    Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas12a
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210035-plasmid-map-sequence-id-413996

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  5. AWP-029

    Plasmid
    #213966
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.
    Depositor
    Alexander Westermann
    Publication
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Segatella bryantii, Synthetic)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213966-plasmid-map-sequence-id-429164

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  8. AWP-031

    Plasmid
    #213967
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.
    Depositor
    Alexander Westermann
    Publication
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Segatella bryantii, Synthetic)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213967-plasmid-map-sequence-id-429166

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  13. pCSN067

    Plasmid
    #101748
    Purpose
    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Publication
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101748-plasmid-map-sequence-id-199897

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Showing: 101 - 120 of 1112 results