We narrowed to 4,064 results for: gast

1. Or63a-Gal4

   Plasmid

   #63043

   Purpose

   To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or63a, using the promoter sequences of the gene cloned 5' of Gal4

   Depositor

   Leslie Vosshall

   Article

   Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

   Insert

   Drosophila melanogaster Or63a promoter
   

   Tags

   Drosophila ADH (aa's 1-18) fused to Yeast Ga…

   Expression

   Insect

   Available Since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAc5.1A-Dm-mir-6-1,2,3

   Plasmid

   #22789

   Depositor

   Elisa Izaurralde

   Article

   Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

   Insert

   micro RNA 6-1, micro RNA 6-2, micro RNA 6-3 (mir-6-1 primary transcript, Fly)
   

   Use

   Drosophila expression

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASTattB-2xFlag-mCherry-Msp300KASH

   Plasmid

   #170807

   Purpose

   Expresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesis

   Depositor

   Vikki Weake

   Article

   Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

   Insert

   2xFLAG/mCherry-Msp300KASH
   

   Tags

   2xFLAG-mCherry and Msp300KASH

   Expression

   Insect

   Promoter

   5xUAS + hsp70 minimal promoter

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCh-139

   Plasmid

   #92287

   Purpose

   Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cells

   Depositor

   Witold Filipowicz, Marina Chekulaeva

   Article

   Chekulaeva et al Nucleic Acids Res. 2010 Oct;38(19):6673-

   Insert

   dGW182 NED (gw Fly)
   

   Tags

   NHA

   Expression

   Insect

   Mutation

   dGW182 NED: aa 205-490

   Promoter

   Ac5

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1A-Dm-miR-13b-1

   Plasmid

   #22762

   Depositor

   Elisa Izaurralde

   Article

   Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

   Insert

   micro RNA 13b-1 (mir-13b-1 primary transcript, Fly)
   

   Use

   Drosophila expression

   Available Since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. helicase cDNA

   Plasmid

   #39552

   Depositor

   Norbert Perrimon

   Article

   Eberl et al Genetics. 1997 Jul;146(3):951-63.

   Insert

   helicase (Hel25E Fly)
   

   Available Since

   Jan. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

7. PRE-Hsp70BbCas9_1.0

   Plasmid

   #190795

   Purpose

   PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   PRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMCh-76

   Plasmid

   #92288

   Purpose

   Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

   Depositor

   Roy Parker, Marina Chekulaeva

   Article

   Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

   Insert

   dGW182 CED (gw Fly)
   

   Tags

   NHA

   Expression

   Insect

   Mutation

   dGW182 CED: aa 940-1215

   Promoter

   Ac5

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRE-Hsp70BbCas9_1.3

   Plasmid

   #190797

   Purpose

   aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Expression

   Insect

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Renilla luciferase-Pol III

    Plasmid

    #37380

    Depositor

    Norbert Perrimon

    Article

    Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

    Insert

    PolIII-Renilla control reporter (RpIII128 Fly)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    RNA PolIII 128 subunit (RpIII128)

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pCB15

    Plasmid

    #194756

    Purpose

    GluRIIA-Rescue-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Mutation

    none

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PRE-Hsp70BbCas9_1.2

    Plasmid

    #190796

    Purpose

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Expression

    Insect

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB19

    Plasmid

    #194757

    Purpose

    EGFP-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    EGFP-Tag

    Mutation

    none

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCB20

    Plasmid

    #194758

    Purpose

    ALFA-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    ALFA-Tag

    Mutation

    none

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUAST-HA, Pbl-A

    Plasmid

    #69792

    Purpose

    Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    van Impel et al Development. 2009 Mar;136(5):813-22. doi

    Insert

    Pebble - Drosophila guanine nucleotide exchange factor
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA tag

    Expression

    Insect

    Mutation

    Encodes Pbl (NP_729306.1) lacking last 9 amino ac…

    Promoter

    hsp70 promoter

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAc5.1B-EGFP-DmAGO2-RC_B

    Plasmid

    #145980

    Purpose

    Insect Expression of DmAGO2-RC

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

    Insert

    DmAGO2-RC (AGO2 Fly)
    

    Expression

    Insect

    Mutation

    A deletion of AA 51-56 and an insertion of 23 AA …

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUAST-HA, Pbl-A DH domain

    Plasmid

    #69794

    Purpose

    Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    van Impel et al Development. 2009 Mar;136(5):813-22. doi

    Insert

    Pebble DH domain
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA-Tag

    Expression

    Insect

    Mutation

    Contains only the Pebble DH domain

    Promoter

    hsp70

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAc5.1B-lambdaN-HA-DmAGO2-RC_B

    Plasmid

    #145981

    Purpose

    Insect Expression of DmAGO2-RC

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

    Insert

    DmAGO2-RC (AGO2 Fly)
    

    Expression

    Insect

    Mutation

    A deletion of AA 51-56 and an insertion of 23 AA …

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMCh-623

    Plasmid

    #92266

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 6W6A (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced W23…

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCh-695

    Plasmid

    #92263

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 PAM2 mut (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced: EF…

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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