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Plasmid#170329PurposeExpresses RORg isoform 2 with N-terminal HisSUMO tag in bacteriaDepositorInsertRORG isoform 2 (RORC Human)
UseTagsHisSUMOExpressionBacterialMutationcodon optimizedPromoterT7Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC/CURT_fluoB
Plasmid#117997PurposeExpression of CURT_fluoB controlled by the Gal1 promoterDepositorInsertCURT_fluoB
UseYeast/bacteria binary vectorTagsExpressionYeastMutationPromoterGal1Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEsg1-6K-Luc
Plasmid#15394DepositorInsertUpstream region of mouse Esg1 gene (Dppa5a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-PES-H1
Plasmid#218704PurposeEncodes codon optimized PES-H1 enzyme for bacterial expressionDepositorInsertPES-H1
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
UseTagsExpressionBacterialMutationG160S- please see depositor commentsPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC/CURT_fluoD
Plasmid#118001PurposeExpression of CURT_flouD controlled by the Gal1 promoterDepositorInsertCURT_flouD
UseYeast/bacteria binary vectorTagsExpressionYeastMutationPromoterGal1Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEsg1-ps-6K-Luc
Plasmid#15395DepositorInsertUpstream region of mouse Esg1 pseudogene
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pESdelta 799
Plasmid#13042DepositorInsertMutS
UseTagsHis6ExpressionBacterialMutationC-terminal deletion from aa 800 through 853.PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pESC-leu2d(empty)
Plasmid#20120DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-2A-TurboGFP-PEST
Plasmid#67180PurposeCRISPaint universal donor for gene expression quantification using destabilized TurboGFPDepositorInsertT2A-TurboGFP-PEST
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-NAT-LACap
Plasmid#80763PurposeExpresses truncated L-A capsid for curing satellite virusDepositorInsertL-A cDNA (L-A Cap)
UseTagsExpressionYeastMutationPromoterPGK1Available sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
PTEF1-yEGFPCLN2PEST-pRS406
Plasmid#64406PurposeTEF promoter driven GFP expressionDepositorInsertTEF1 promoter (TEF1 Budding Yeast)
UseTagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaD (pESC106)
Plasmid#50388PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertPPAT
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaE (pESC124)
Plasmid#50390PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertDPCK
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSAD-F3-NPEST-mCherry
Plasmid#104332PurposeSelf-inactivating DG-Rabies (SiR) expressing mCherryDepositorInsertmCherry
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1-Hypa
Plasmid#218166PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mVenus-RapR-PTPPest
Plasmid#188657PurposeRapamycin regulated PTPPest with mVenus tagDepositorInsertProtein Tyrosine Phosphatase Pest (PTPN12 )
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-hPOLISc
Plasmid#60819Purposeto express human Pol iota recoded to yeast codons in yeastDepositorInsertPOLI (POLI Human)
UseTagsc-mycExpressionYeastMutationoptimized to yeast codon usagePromoterGAL1Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-PmCDA1
Plasmid#60818Purposeto overexpress lamprey deaminase in yeastDepositorInsertCDA1
UseTagsFLAGExpressionYeastMutationPromoterGAL10Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-HsAIDSc
Plasmid#60810Purposeto express human AID recoded for yeast codon usageDepositorInsertAID (AICDA Human)
UseTagsMycExpressionYeastMutationcodon optimized for yeastPromoterGAL1Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only