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Plasmid#184469PurposeFor gateway cloning of PARP11DepositorInsertPARP11 (PARP11 Human)
UseTagsExpressionMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1–eGFP
Plasmid#211552PurposeA piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
SFRP1A-c500
Plasmid#205122PurposeSFRP1 protein expressionDepositorInsertSFRP1 (SFRP1 Human)
UseTagsAviTag-6His and PTPRSspExpressionMammalianMutationWTPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFRP1A-c501
Plasmid#205123PurposeSFRP1 protein expressionDepositorInsertSFRP1 (SFRP1 Human)
UseTagsAviTag-6His and PTPRSspExpressionMammalianMutationWTPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-509
Plasmid#209800PurposeBacterial expression of human LARP1 La-moduleDepositorInsertLARP1 La-module (LARP1 Human)
UseTags6xHisExpressionBacterialMutationaa 323-509 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-410
Plasmid#209801PurposeBacterial expression of human LARP1 LaM domainDepositorInsertLARP1 LaM (LARP1 Human)
UseTags6xHisExpressionBacterialMutationaa 323-410 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hARFRP1(WT)-HA
Plasmid#206399PurposeMammalian expression of hARFRP1DepositorInsertARFRP1 (ARFRP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2delBRCT
Plasmid#173939PurposeBacterial expression of PARP1 lacking Zn2 and BRCT domainsDepositorInsertPARP1delZn2delBRCT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 97-213 and 374-520; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Prp16
Plasmid#111351Purposeprotein purificationDepositorInsertPRP16
UseTagsN-terminally 10xHis-tagged proteins with a Factor…ExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
HM110 prp16-2
Plasmid#111277Purposeprp16-2 in pSE358(Trp)DepositorInsertPRP16
UseTagsExpressionYeastMutationprp-2 (D575N)PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB58 (Prp16)
Plasmid#111282PurposePrp16 in pSE358(Trp)DepositorInsertPRP16
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-PRP11
Plasmid#87594PurposeEncodes Y2H Gal4 DNA binding domain for Prp11DepositorInsertPRP11 (PRP11 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Rrp1
Plasmid#63773Purposeyeast ORF Rrp1 in the Gateway entry vector pDONR221DepositorInsertRrp1
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMV-TRP1
Plasmid#65335Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUsDepositorInsertTRP1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARFRP1
Plasmid#67387PurposeExpression of native hArfrp in E. coliDepositorInsertARFRP1 (ARFRP1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARFRP1
Plasmid#67342PurposehArfrp1 with stop codon in Gateway Entry vectorDepositorInsertARFRP1 (ARFRP1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
hPrp16 N-terminus
Plasmid#11204DepositorInsertPrp16 N-terminus (DHX38 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 1-554PromoterAvailable sinceJan. 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
hPrp16 C-terminus
Plasmid#11205DepositorInsertPrp16 C-terminus (DHX38 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 948-1227PromoterAvailable sinceJan. 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-TRP1URA3
Plasmid#11008DepositorInsertTRP1URA3 fusion
UseTagsV5 and his6ExpressionBacterial and YeastMutationORF-TRP1 fused to ORF-URA without stop codonPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-1
Plasmid#89972Purposeprp16-1 allele in TRP plasmidDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationprp16-1 allele (Y386D)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only