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CTNNB1 - W388S in pENTR

Plasmid

#216618

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Mutation

W388S

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCtnnd2.2-GFP

Plasmid

#209098

Purpose

GFP expressing scramble of shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.2

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCtnnd2.2-GFP

Plasmid

#209097

Purpose

GFP expressing shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.2

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCtnnd2.1-GFP

Plasmid

#209096

Purpose

GFP expressing scramble of shRNA targeting Ctnnd2 N terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.1

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRepair-mTq2-CTNNB1

Plasmid

#153433

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

mTurquoise2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-CTNNB1_S33Y

Plasmid

#133376

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

CTNNB1 (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S33Y

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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TNNI3K gRNA (BRDN0001487146)

Plasmid

#77896

Purpose

3rd generation lentiviral gRNA plasmid targeting human TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (TNNI3K, FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TNNI3K gRNA (BRDN0001146965)

Plasmid

#77897

Purpose

3rd generation lentiviral gRNA plasmid targeting human TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (TNNI3K, FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TNNI3K gRNA (BRDN0001147407)

Plasmid

#77898

Purpose

3rd generation lentiviral gRNA plasmid targeting human TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K, TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pUC118-mCtnnb1-FLIP-FlpE-Puro

Plasmid

#84541

Purpose

CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mouse beta-catenin FLIP-FlpE - Puro targeting vector

Use

Cre/Lox and Mouse Targeting

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pAAVS1-Puro-TNNT2-Neo

Plasmid

#214013

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_Wild-type

Plasmid

#234551

Purpose

Human a-Ecat sequence/CTNNA1

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_Wild-type (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

None

Promoter

RSV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pUC118-mCtnnb1-FLIP-Puro

Plasmid

#84540

Purpose

CRISPR - FLIP targeting vector for mouse beta-catenin

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mouse beta-catenin FLIP - Puro targeting vector

Use

Cre/Lox and Mouse Targeting

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pT2/GD-IRES-GFP-CTNNB1

Plasmid

#192868

Purpose

Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

Depositor

David Largaespada

Article

Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

Inserts

luc+
EGFP
CTNNB1

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Puro

Plasmid

#214016

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Zeo

Plasmid

#214017

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_H0-ABD+

Plasmid

#234552

Purpose

CTNNA1 enhanced-actin-binding domain mutations

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_H0-ABD+ (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

R666G, A667S, I668G, M669S

Promoter

CMV

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-delta1-3-HA

Plasmid

#242219

Purpose

Expression of human β-catenin ∆arm 1-3 (deletion of a.a. 134-266) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted arm 1-3 from β-catenin (deletion of a.a. …

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-Armadillo-HA

Plasmid

#242224

Purpose

Expression of human β-catenin Armadillo repeats (deletion of a.a. 2-133 and a.a. 665-781) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted N terminal and C terminal IDRs from β-cat…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-deltaC-HA

Plasmid

#242223

Purpose

Expression of human β-catenin ∆C terminal IDR (deletion of a.a. 665-781) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted C terminal IDR from β-catenin (deletion o…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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