We narrowed to 981 results for: transfection

1. phOct4-EGFP

   Plasmid

   #38776

   Depositor

   Wei Cui

   Article

   Gerrard et al Stem Cells. 2005;23(1):124-33.

   Insert

   Oct-4 promoter (POU5F1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   human OCT4

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTC-ApoE-Tet-YAP

   Plasmid

   #85580

   Purpose

   Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liver

   Depositor

   Ursula Ehmer

   Article

   Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

   Inserts

   CreER
   YAP
   

   Use

   Cre/Lox

   Tags

   Flag (2x)

   Expression

   Mammalian

   Promoter

   ApoE.HCR.hAAT and TRE

   Available Since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTC-CMV-Tet-YAP

   Plasmid

   #85579

   Purpose

   Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (CMV promoter ) in mouse liver

   Depositor

   Ursula Ehmer

   Article

   Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

   Inserts

   CreER
   YAP
   

   Use

   Cre/Lox

   Tags

   Flag (2x)

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pONSY-Lifeact:mCherry

   Plasmid

   #111876

   Purpose

   This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Lifeact fused to mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-rat Fcepsilon RI gamma

   Plasmid

   #8375

   Depositor

   Jean Kinet

   Article

   Blank et al Nature 1989 Jan 12;337(6203):187-9.

   Insert

   rat Fc epsilon RI gamma cDNA (Fcer1g Rat)
   

   Use

   Beta-gal

   Expression

   Bacterial

   Available Since

   June 7, 2005

   Availability

   Academic Institutions and Nonprofits only

6. YFP-cCGNL1-Myc

   Plasmid

   #236513

   Purpose

   For expression of Paracingulin (dog) in mammalian cells

   Depositor

   Sandra Citi

   Article

   Paschoud et al Mol Membr Biol. 2008 Jan;25(1):1-13. doi

   Insert

   CGNL1 (CGNL1 Canis lupus familiaris (dog))
   

   Tags

   Myc and YFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAHW/Joseph2-Multitagged Plasmid

   Plasmid

   #226725

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pAHW/Joseph2
   

   Use

   Drosophila s2 cell expression

   Tags

   HA, Myc, GFP, Flag, V5, 6xHis

   Expression

   Insect

   Promoter

   Act5C

   Available Since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Clim-DC2-lifeact-mCherry

   Plasmid

   #129558

   Purpose

   pClimDCLifeAct:mCh actin filaments marker

   Depositor

   Inaki Ruiz-Trillo

   Article

   Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

   Insert

   lifeAct fused to mCherry
   

   Expression

   Bacterial

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTM

   Plasmid

   #162783

   Purpose

   Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded

   Depositor

   Ivan Vorobiev

   Article

   Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

   Type

   Empty backbone

   Tags

   10xHis, c-myc, and hTPA leader

   Expression

   Mammalian

   Promoter

   CHO Elongation factor 1A

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Clim-DC2-NMM-tdtomato

    Plasmid

    #129557

    Purpose

    pClimDC-NMM:tdT cell membrane marker

    Depositor

    Inaki Ruiz-Trillo

    Article

    Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

    Insert

    N-Myristoylation motif (NMM) fused to tdtomato
    

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBJ1 neo-mo FceRI beta

    Plasmid

    #8604

    Depositor

    Jean Kinet

    Article

    Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

    Insert

    mouse Fc epsilon RI beta cDNA (Ms4a2 Mouse)
    

    Expression

    Mammalian

    Available Since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

12. Clim-DC2-H2B2-Venus

    Plasmid

    #129556

    Purpose

    pClimDC-H2B:mV nuclear marker

    Depositor

    Inaki Ruiz-Trillo

    Article

    Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

    Insert

    Histone 2B fused to venus
    

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDOE20 mC VN transient

    Plasmid

    #124247

    Purpose

    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.

    Depositor

    Attila Fehér

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGFP-LacI

    Plasmid

    #179510

    Purpose

    Expresses EGFP-LacI in mammalian cells

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    LacI
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSVL-mo FceRI gamma

    Plasmid

    #8372

    Depositor

    Jean Kinet

    Article

    Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

    Insert

    mouse Fc epsilon RI gamma cDNA (Fcer1g Mouse)
    

    Expression

    Mammalian

    Available Since

    June 7, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pONSY-CoNMM:mCherry

    Plasmid

    #111878

    Purpose

    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p1.1-Tr2-eGFP

    Plasmid

    #162782

    Purpose

    Fluorescent reporter for protein expression studies

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Insert

    enhanced green fluorescent protein
    

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. hsLEMD2-pX330

    Plasmid

    #179511

    Purpose

    Encodes gRNA for human LEMD2

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    gRNA for LEMD2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNHT7

    Plasmid

    #186753

    Purpose

    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Type

    Empty backbone

    Use

    Synthetic Biology; Cloning backbone

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMYH9-WT-V5

    Plasmid

    #183512

    Purpose

    Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Insert

    MYH9 (MYH9 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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