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pTMB340_ZFcharm_Kv1_Prnp_DPM

Plasmid

#220843

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1505

Plasmid

#62359

Purpose

RPL16a HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPL16A

Use

Yeast tagging

Tags

HA-TEV-AVI

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1+ HA-hAO7

Plasmid

#137840

Purpose

N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Tags

HA

Expression

Mammalian

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-Nedd4-C / 2523 JM15

Plasmid

#13311

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1-422 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pLG_GI4

Plasmid

#111595

Purpose

GI Library Vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-Nedd4-N / 2524 JM16

Plasmid

#13312

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa1-52 and aa422-777 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pLG_GI2

Plasmid

#111593

Purpose

mU6-sgRNA GI Library vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLG_GI3

Plasmid

#111594

Purpose

hU6-sgRNA GI Library vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6p1 AO7RE

Plasmid

#137843

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-gp78 R2M / JM21

Plasmid

#13304

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Expression

Mammalian

Mutation

C356G ; H362A

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pJW1665

Plasmid

#112039

Purpose

Integrating plasmid for expression of ER localized mCherry at TRP1 locus

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ER-mCherry

Expression

Yeast

Promoter

TPI

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-MmUbc7/2776

Plasmid

#11488

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc7 (Ube2g2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

n/a

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pET 15b-UbcH5c/2418

Plasmid

#11473

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5c (UBE2D3 Human)

Expression

Bacterial

Mutation

n/a

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pJW1667

Plasmid

#112041

Purpose

CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ERG5

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1660

Plasmid

#112034

Purpose

C-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEU

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

IAA17

Tags

HA

Expression

Yeast

Mutation

only amino acids 71-114

Promoter

None

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1510

Plasmid

#62364

Purpose

pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ubc6 tail anchor

Expression

Yeast

Mutation

L232H mutation compared to standard mVenus

Promoter

Sec63

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-HA-MmUbc6/2774

Plasmid

#11482

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc6 (Ube2j2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

n/a

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-4T-3-TRC8 513-664

Plasmid

#185352

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

TRC8 (RNF139 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 513-664 only

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HRD1 aa 236-444

Plasmid

#185349

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

HRD1/SYVN (SYVN1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 236-444 only

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET3a-UBE2J1 1-283

Plasmid

#185348

Purpose

In vitro transcription and translation in bacterial system

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

UBE2J1 (UBE2J1 Human)

Expression

Bacterial

Mutation

Cytoplasmic domain only, aa 1-283

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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