168,238 results

1. pET263-pUC57 24xMS2V7

   Plasmid

   #140705

   Purpose

   MS2V7 cloning

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V7
   

   Use

   Cloning vector

   Mutation

   24 MS2 loops V7, high-affinity C-variant, inter-s…

   Promoter

   None

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-PE7 for IVT

   Plasmid

   #214813

   Purpose

   Template for in vitro transcription of PE7

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Promoter

   T7 (inactivated)

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCXWB-EBNA1

   Plasmid

   #37624

   Purpose

   Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   EBNA-1 (EBNA-1 human herpesvirus 4)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBOB-EF1-FastFUCCI-Puro

   Plasmid

   #86849

   Purpose

   3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

   Depositor

   Kevin Brindle, Duncan Jodrell

   Article

   Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

   Inserts

   mKO2-hCDT1(30-120)
   mAG-hGEM(1-110)
   pac (puromycin N-acetyl-transferase)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1 and PGK

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP1339

   Plasmid

   #119271

   Purpose

   for cloning new sgRNAs

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PE6d

   Plasmid

   #207854

   Purpose

   Mammalian expression of PE6d prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6d
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIX_403

   Plasmid

   #41395

   Purpose

   Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   V5

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9

   Plasmid

   #42876

   Purpose

   Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   tracr/Cas9
   

   Use

   CRISPR; E.coli

   Expression

   Bacterial

   Available Since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV1)

   Viral Prep

   #104492-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

10. BK Dunlop

    Plasmid

    #25466

    Depositor

    Peter Howley

    Insert

    human papovavirus BK Dunlop
    

    Expression

    Bacterial

    Available Since

    Sept. 26, 2011

    Availability

    Academic Institutions and Nonprofits only