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Plasmid#123209PurposeGateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C no-stop
Plasmid#123208PurposeGateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP no-stop
Plasmid#123209PurposeGateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C no-stop
Plasmid#123208PurposeGateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA1 Q1779Stop
Plasmid#139331PurposePlasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editingDepositorInsertsgRNA to insert BRCA1 Q1779Stop using base editing
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA1 Q1779Stop
Plasmid#139331PurposePlasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editingDepositorInsertsgRNA to insert BRCA1 Q1779Stop using base editing
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2 no stop
Plasmid#80806Purposered fluorophore without stop codonDepositorInsertpME-mKate2 no stop
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2 no stop
Plasmid#80806Purposered fluorophore without stop codonDepositorInsertpME-mKate2 no stop
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nluc(W12stop)
Plasmid#180502PurposeMammalian expression of Nluc(W12stop)DepositorInsertNluc(W12stop)
UseTagsFLAGExpressionMammalianMutationW12(UGG) to amber stop codon(UAG)PromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nluc(W12stop)
Plasmid#180502PurposeMammalian expression of Nluc(W12stop)DepositorInsertNluc(W12stop)
UseTagsFLAGExpressionMammalianMutationW12(UGG) to amber stop codon(UAG)PromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-STOP-shA1
Plasmid#14422DepositorInsertmodified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
UseCre/Lox and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pU6-STOP-shA1
Plasmid#14422DepositorInsertmodified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
UseCre/Lox and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-STOP
Plasmid#66107PurposeThis vector attaches YFP(1-158) to the C-terminus of a proteinDepositorInsertYFP(1-158)STOP
UseTagsExpressionMammalianMutationGln69 was changed to Met.PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-STOP
Plasmid#66107PurposeThis vector attaches YFP(1-158) to the C-terminus of a proteinDepositorInsertYFP(1-158)STOP
UseTagsExpressionMammalianMutationGln69 was changed to Met.PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARFRP1-no stop
Plasmid#67343PurposehArfrp1 without stop codon in Gateway Entry vectorDepositorInsertARFRP1 (ARFRP1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARFRP1-no stop
Plasmid#67343PurposehArfrp1 without stop codon in Gateway Entry vectorDepositorInsertARFRP1 (ARFRP1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV FHA-Stop
Plasmid#27050DepositorInsertautophagy-related 12 (yeast) (Atg12 Mouse)
UseRetroviralTagsHA and flagExpressionMammalianMutationchanged glycine 141 to stop codon.PromoterAvailable sinceJan. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCV FHA-Stop
Plasmid#27050DepositorInsertautophagy-related 12 (yeast) (Atg12 Mouse)
UseRetroviralTagsHA and flagExpressionMammalianMutationchanged glycine 141 to stop codon.PromoterAvailable sinceJan. 13, 2011AvailabilityAcademic Institutions and Nonprofits only