We narrowed to 4,940 results for: mpo

1. pAuroraA-GFP-AURKA_K162M-mCherry

   Plasmid

   #99880

   Purpose

   Expression of GFP-AURKA K162M-mCherry in cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP and mCherry

   Expression

   Mammalian

   Mutation

   K162M (kinase-dead)

   Promoter

   AURKA minimal

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAL113

   Plasmid

   #22471

   Purpose

   Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB NT (PHYB Mustard Weed, Mouse)
   

   Use

   Synthetic Biology

   Tags

   10aaLinker-mCFP-HrasCT

   Expression

   Mammalian

   Mutation

   Y276H

   Promoter

   CMV

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV N-term HA-FLAG puro BRD7

   Plasmid

   #41934

   Depositor

   Stephen Elledge

   Article

   Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   BRD7 (BRD7 Human)
   

   Use

   Retroviral

   Tags

   HA-Flag

   Expression

   Mammalian

   Available Since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pDEST47-MCU-GFP

   Plasmid

   #31732

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   *

   Promoter

   CMV

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. CD8-mCherry-FRB

   Plasmid

   #100738

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD8A (CD8A Human)
   

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Mutation

   Truncated

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRX-IP/SECFP-hATG16A1

   Plasmid

   #58994

   Purpose

   Expresses SECFP-hATG16A1 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)
   

   Use

   Retroviral

   Tags

   SECFP

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p415 TEF Su9‐roGFP2‐Tsa2ΔCR

   Plasmid

   #83240

   Purpose

   The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

   Insert

   Su9-roGFP2-Tsa2dCr
   

   Tags

   roGFP2 fusion with Tsa2dCr with Su9 targetting se…

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP-epsin(144-575)-GFP

   Plasmid

   #100733

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   epn1 (Epn1 Mouse)
   

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   clathrin binding middle part aa 144-575

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH-SFB-BRCA1

   Plasmid

   #99394

   Purpose

   Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1

   Depositor

   Junjie Chen, Michael Huen

   Article

   Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C2-Cep123-C2 domain

    Plasmid

    #73329

    Purpose

    Encodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

    Insert

    Cep123 (CEP89 Human)
    

    Tags

    EGFP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAd-CCN1

    Plasmid

    #12519

    Depositor

    Tong-Chuan He

    Article

    Si et al Mol Cell Biol. 2006 Apr . 26(8):2955-64.

    Insert

    CCN1 (Ccn1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-hSynapsin- soCoChR-GFP

    Plasmid

    #107708

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Has Service

    AAV9

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLM-mCitrine-Sox2

    Plasmid

    #23242

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCitrine_2A_SOX2 (SOX2 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-IP/Venus-rLC3

    Plasmid

    #58740

    Purpose

    Expresses Venus-rLC3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pDONR223-MCU-D261A

    Plasmid

    #31729

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Mutation

    Changed D261 to Alanine

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-MCU-E264A

    Plasmid

    #31730

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Mutation

    Changed E264 to Alanine

    Promoter

    None

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1alpha-soCoChR-tdTomato

    Plasmid

    #107714

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-tdTomato
    

    Use

    AAV

    Tags

    KA2 and tdTomato

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-GFP-AURKA_K162M-mCherry

    Plasmid

    #99881

    Purpose

    Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    K162M (kinase-dead)

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-MCU-E257A

    Plasmid

    #31727

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Mutation

    Changed E257 to Alanine

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-MTS-WT-c-Src-FLAG

    Plasmid

    #44652

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Tags

    FLAG tag and mitochondria-targeting sequence (res…

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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