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We narrowed to 63,901 results for: cloning

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Showing: 11981 - 12000 of 63901 results

  1. Hs.KRAS4a Q61R

    Plasmid
    #83172
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61R
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83172-plasmid-map-sequence-id-158135

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  2. Hs.KRAS4b Q61R

    Plasmid
    #83135
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61R
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83135-plasmid-map-sequence-id-158098

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  7. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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  8. pX461-ABE8e-nSpCas9

    Plasmid
    #237460
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_ABE8e_SpCas9(D10A)_6xNLS
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237460-plasmid-map-sequence-id-476159

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  9. pX462-ABE8e-nSaCas9

    Plasmid
    #237462
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSaCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_ABE8e_SaCas9 -6xNLS
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237462-plasmid-map-sequence-id-476147

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  12. p3015b

    Plasmid
    #209317
    Purpose
    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.
    Depositor
    Thomas Hooven
    Publication
    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1
    Insert
    Group B Streptococcus-compatible single guide RNA
    Expression
    Bacterial
    Promoter
    Xyl/tet promoter that is constitutively active
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    209317-plasmid-map-sequence-id-435309

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  13. pMDC32B-BS-AtMIR390a-B/c

    Plasmid
    #199560
    Purpose
    Plant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stem
    Depositor
    Alberto Carbonell
    Publication
    Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10
    Insert
    Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
    Use
    RNAi
    Promoter
    2x35S
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    199560-plasmid-map-sequence-id-398170

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  14. P[acman]-Alpha2

    Plasmid
    #165859
    Purpose
    Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Publication
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165859-plasmid-map-sequence-id-336455

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Showing: 11981 - 12000 of 63901 results