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pFastBacDual with LFn + Fla

Plasmid

#84866

Purpose

Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Use

Baculovirus

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEF Itk myc

Plasmid

#20705

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk (Itk Mouse)

Tags

myc

Expression

Mammalian

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
pgex-cIAP1 mut

Plasmid

#8334

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

ciap1mut (BIRC2 Human)

Tags

GST

Expression

Bacterial

Mutation

H588A

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only
pETpRha-alpha-SkTm

Plasmid

#72929

Purpose

Allows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoter

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha skeletal Tropomyosin

Expression

Bacterial

Promoter

rhaB

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSR47

Plasmid

#69258

Purpose

pmyo-3::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

myo-3

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIK3R5

Plasmid

#23819

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3R5 (PIK3R5 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pRS314-hUD

Plasmid

#11005

Depositor

Francisco Malagon

Insert

hUD recombination system

Expression

Bacterial and Yeast

Mutation

Direct repeat recombination system. Recombination…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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Anti-VIP [L108/92R]

Plasmid

#177453

Purpose

Mammalian Expression Plasmid of anti-VIP (Human). Derived from hybridoma L108/92.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VIP (Homo sapiens) recombinant mouse monoclonal antibody (VIP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRAF4

Plasmid

#58318

Purpose

EGFP-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MKK1

Plasmid

#107973

Purpose

expression of MAP Kinase Kinase

Depositor

Edwin Krebs

Article

Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

Insert

MKK1 (MAP2K1 Human)

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP1-S163A

Plasmid

#18989

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein S163A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Serine 163 changed to Alanine,

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pLS1/-11

Plasmid

#31492

Depositor

Kathleen Matthews

Insert

Lactose repressor with 11 amino acid C-terminal deletion (lacI E. coli variant)

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pAd5-B1-deltaE1-MCS

Plasmid

#122558

Purpose

Modified block 1, with deletion in E1

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Mutation

Adenovirus sequences 481-3530 replaced with multi…

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-CRM197-4xDQNAT

Plasmid

#128403

Purpose

Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Diphtheria toxin

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Inactivating G52E mutation

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:GFPNLS-SV40pA

Plasmid

#155123

Purpose

Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe neo TGFBR3-HA

Plasmid

#83095

Purpose

Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

TGFBR3 (TGFBR3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLostfoX-Kan

Plasmid

#99865

Purpose

TfoX induction vector for chitin-pathway transformation (KanR)

Depositor

Mark Mandel

Article

Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

Insert

tfoXVF

Use

V. fischeri suicide cloning vector, cmr, tcr

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLEXm-GST-ATG14

Plasmid

#99329

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

ATG14 (ATG14 Human)

Tags

GST-TEV tag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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BPTF PHD and Bromodomain

Plasmid

#92101

Purpose

Expresses GST fusion PHD and Bromodomain from BPTF protein

Depositor

Albert Jeltsch

Article

Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

Insert

PHD and Bromodomain (BPTF Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK7 T194D

Plasmid

#80886

Purpose

mammalian expression of ALK7 T194D

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

ALK7 (Acvr1c Rat)

Tags

HA

Expression

Mammalian

Mutation

T194D (constitutively active)

Promoter

CMV

Available Since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only