165,813 results

1. pCryptDel4.8

   Plasmid

   #141293

   Purpose

   Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Inserts

   RelB
   gRNA targetting pMUT2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Native promoter from pMUT2 relB/relE operon and P…

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3 Flag Jnk3a1

   Plasmid

   #13758

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk3a1 (MAPK10 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-dCas13-inactive M3M14nes

   Plasmid

   #157855

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LCV2_LacZ_sgRNA_2

   Plasmid

   #155093

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-H2B-Venus

   Plasmid

   #87614

   Purpose

   Venus (YFP) fused to histone H2B.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   H2B-Venus (H2BC8 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HA-NLGN4 WT

   Plasmid

   #59320

   Purpose

   Expression plasmid of HA-tagged wildtype human Neuroligin 4

   Depositor

   Peter Scheiffele

   Article

   Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

   Insert

   NLGN4 (NLGN4X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGM4723:Ble:Cas9

   Plasmid

   #112208

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

   Depositor

   Thomas Mock

   Inserts

   Cas9
   SheBle
   

   Use

   CRISPR; Diatom, phaeodactylum

   Tags

   Phaeodactylum fcp promoter and terminator and YFP

   Expression

   Mutation

   Phaeodactylum Fcp promoter

   Promoter

   Phaeodactylum Fcp

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5_mEGFP-EWS-FLI1

   Plasmid

   #237636

   Purpose

   For overexpression of mEGFP-EWS-FLI1

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EWS-FLI1 (EWSR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3998 pGEX HHR23A

    Plasmid

    #10864

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem. 1999 Jun 25. 274(26):18785-

    Insert

    HHR23A (RAD23A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only