163,421 results

1. pCAG-MBP-ATG9

   Plasmid

   #188076

   Purpose

   Recombinant overexpression of human ATG9 in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Use

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFX-iRFP-Rab7

   Plasmid

   #174459

   Purpose

   Express iRFP with late endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with late endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab7)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CAG-jGCaMP8f-WPRE (AAV9)

   Viral Prep

   #179254-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CAG

   Tags

   None

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

4. AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) (AAV PHP.eB)

   Viral Prep

   #199776-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) (#199776). In addition to the viral particles, you will also receive purified AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) plasmid DNA. Cre recombinase expression in striatal direct pathway medium spiny neurons (D1-MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Promoter

   Tags

   None

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

5. pSIP403

   Plasmid

   #122028

   Purpose

   Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

   Depositor

   Geir Mathiesen, Lars Axelsson

   Article

   Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

   Insert

   gusA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sppA

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-myc-GLUT4-mCherry

   Plasmid

   #64049

   Purpose

   3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   GLUT4 (Slc2a4 Mouse)
   

   Use

   Lentiviral

   Tags

   c-Myc and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

   Plasmid

   #118617

   Purpose

   Lentiviral expression of mTurq2 tagged PCNA

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   TK-NLS-mTurq2-PCNA (PCNA Human)
   

   Use

   Lentiviral

   Tags

   mTurq2

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

   Viral Prep

   #112677-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

    Plasmid

    #67986

    Purpose

    Cas9 activity reporter with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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