Addgene

We narrowed to 1,962 results for: IPP

Showing: 1201 - 1220 of 1962 results

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pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126698

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

sgRNA targeting human SCGB3A2 locus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-3xFLAG-YAP1-S127A

Plasmid

#42239

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YAP1 (YAP1 Human)

Use

Gateway

Tags

3xFLAG

Expression

Mutation

Serine 127 changed to alanine

Promoter

none

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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YA1532: pAAV_ CAG-FLEX-paQuasAr3

Plasmid

#107702

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-GW-A

Plasmid

#138480

Purpose

For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-TEV-U1AF37MF77M

Plasmid

#168267

Purpose

Expresses human dmU1A with the F37M/F77M double mutant

Depositor

Joseph Wedekind

Article

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

U1A RRM1 crystallization module (SNRPA Human)

Use

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F37M and F77M

Promoter

T7 promotor

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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rAAV-U6-MeCP2shRNA-CamKIIa-mCherry

Plasmid

#169704

Purpose

MeCP2 Knockdown

Depositor

Ana Oliveira

Article

Gulmez Karaca et al Neurobiol Learn Mem. 2018 Mar;149:84-97.

Insert

shRNA against MeCp2 (Mecp2 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ_VNp-LZ-hGH

Plasmid

#182412

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ-hGH (GH1 Human)
VNp-LZ

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MAPK14-P2A-Hygro_Barcode

Plasmid

#170239

Purpose

Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAPK14 (MAPK14 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-del C

Plasmid

#33095

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

deleted C terminal (amino acids 291-450)

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-AmCyan

Plasmid

#138481

Purpose

For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

AmCyan

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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YA1531: pAAV_CAG-FLEX-QuasAr3

Plasmid

#107701

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-STK3

Plasmid

#172990

Purpose

constitutive expression of FLAG-tagged STK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-STK3 (STK3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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YA1629: pAAV_CAG-FLEX-paQuasAr3-s

Plasmid

#107703

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSCT-GAL93-NONO

Plasmid

#46325

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

NONO (NONO Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
LV_EF1a_CDK1-P2A-Hygro_Barcode

Plasmid

#170221

Purpose

Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK1 (CDK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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tdEos-Vimentin-7

Plasmid

#57688

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Vimentin (VIM Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti HsATP10B - EmGFP

Plasmid

#204474

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B WT fused with EmGFP

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Inserts

ATP10B (ATP10B Human)
EmGFP

Use

Lentiviral

Tags

EmGFP

Expression

Mutation

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

Plasmid

#170222

Purpose

Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Tags

Expression

Mutation

R24C

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1puro-shKIBRA-A

Plasmid

#40888

Depositor

Paul Reynolds

Article

Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

Insert

shKIBRA-A (WWC1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

Plasmid

#170217

Purpose

Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CASP8 (CASP8 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

C360A

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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