Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 16,608 results for: LEA

Showing: 1201 - 1220 of 16608 results

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pCL1002

Plasmid

#252428

Purpose

mCherry-tagged NASP with nuclear localisation sequence (NLS) deleted

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

sNASP-dNLS (NASP Human)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of NLS (residues 378-383)

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCW57.1 DOX off puro RELA

Plasmid

#241440

Purpose

Expresses DOX-suppressible RELA

Depositor

Richard Possemato

Insert

RELA (RELA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Partly codon optimized

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2-mCherry sgmRELA_2

Plasmid

#241453

Purpose

Delete mouse Rela

Depositor

Richard Possemato

Insert

sgmRELA_2 (RELA Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 sgTMEM173_1

Plasmid

#241456

Purpose

Delete TMEM173 (STING)

Depositor

Richard Possemato

Insert

sgTMEM173_1 (STING1 Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 sgTMEM173_2

Plasmid

#241457

Purpose

Delete TMEM173 (STING)

Depositor

Richard Possemato

Insert

sgTMEM173_2 (STING1 Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 hygro sgmPOLE_2

Plasmid

#241443

Purpose

Delete mouse Pole

Depositor

Richard Possemato

Insert

sgmPOLE_2 (POLE Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 hygro sgmPOLE_1

Plasmid

#241442

Purpose

Delete mouse Pole

Depositor

Richard Possemato

Insert

sgmPOLE_1 (POLE Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2-mCherry sgmRELA_1

Plasmid

#241447

Purpose

Delete mouse Rela

Depositor

Richard Possemato

Insert

sgmRELA_1 (RELA Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_TEV_aMFx1 (4a)

Plasmid

#238903

Purpose

Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_TEV_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pTrcHisA-NLS-BFP-mEosEM

Plasmid

#218934

Purpose

Express the nuclear import substrate NLS-BFP-mEosEM.

Depositor

Siegfried Musser

Article

Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

Insert

BFP-mEosEM fusion protein

Tags

6xHis and SV40 nuclear localization sequence (NLS…

Expression

Bacterial

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg6-mut-siRNAres_I

Plasmid

#146588

Purpose

Mammalian Expression of HsSmg6-mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

HsSmg6-mut-siRNAres (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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KBB416

Plasmid

#185087

Purpose

Swi6L-GFP with nuclear export signal mutated to alanines

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 nuclear export signal L to A mutations

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG

Plasmid

#130678

Purpose

Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D409_F489del

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG

Plasmid

#130680

Purpose

Expresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_G1960del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAG

Plasmid

#130677

Purpose

Expresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

M1961_E1992del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG

Plasmid

#130691

Purpose

Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

E653_G1960del

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG

Plasmid

#130689

Purpose

Expresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, M1961_E1992del

Promoter

CMV

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAG

Plasmid

#130686

Purpose

Expresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-9.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I378_G1960del

Promoter

CMV

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS attB V5 dDispatched WT HA

Plasmid

#126411

Purpose

Expression of wild type dDispatched HA in Drosophila.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

Plasmid

#130690

Purpose

Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, G1720_Y1846del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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