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Plasmid#127358Purposegateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Histone H3.3 [N158/28R]
Plasmid#182106PurposeMammalian Expression Plasmid of anti-Histone H3.3 (Rat). Derived from hybridoma N158/28.DepositorInsertanti-Histone H3.3 (Rattus norvegicus) recombinant mouse monoclonal antibody (H3f3b Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS->Bxb1.STOP>myr::4xSNAPf
Plasmid#87639PurposeFor creating drosophila transgenics expressing UAS->Bxb1.STOP>myr::4xSNAPfDepositorInsertmyr::4xSNAPf
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lox-stop-Lox p53 R172H
Plasmid#14854DepositorInsertp53 R172H (Trp53 Mouse)
UseCre/LoxTagsLox-STOP-loxExpressionMammalianMutationStructural mutant R172H. (Murine p53 codon 172 co…PromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-miniTurbo-NES-YFP-STOP-L2
Plasmid#127359Purposegateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choiceDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lox-Stop-Lox TOPO Hygro
Plasmid#35688DepositorInsertloxP-STOP-loxP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP5 Cys146Ala_nostop
Plasmid#154030PurposeGateway-compatible Entry vectorDepositorInsertNSP5
UseGateway-compatible entry vector, with mutagenized…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop
Plasmid#154029PurposeGateway-compatible Entry vectorDepositorInsertNSP3
UseGateway-compatible entry vector, with mutagenized…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
Plasmid#68345PurposeA Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expressionDepositorInsertspuromycin
hCas9
UseCRISPR; Flp/frtTagslinked to red flouresent protein gene mKateII and…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2
Plasmid#186361PurposeEntry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.DepositorInsertHuman Ensconsin (MAP7 Human, Synthetic)
UseExpression of a fluorescent microtubule markerTags3xEGFPExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-GAP43-ECFP-stop-L2
Plasmid#186356PurposeEntry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequencesDepositorInsertGrowth-associated protein-43 (Gap43 Synthetic, Mouse)
UseExpression of a fluorescent membrane markerTagsECFPExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP5002 RabV-SADdeltaG-histone-EGFP
Plasmid#176284PurposeRecombinant rabies virus expressing histone-EGFPDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-EGFP-GPI-stop-L2
Plasmid#186355PurposeEntry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequencesDepositorInsertsignal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Human, Synthetic)
UseExpression of a fluorescent membrane markerTagsEGFPExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lox-stop-Lox p53 R270H
Plasmid#14853DepositorInsertp53 R270H (Trp53 Mouse)
UseCre/LoxTagsLox-STOP-loxExpressionMammalianMutationContact mutant R270H. (Murine p53 codon 270 corre…PromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-EGFP-mouse Plaat3 (N157stop)
Plasmid#168771PurposeExpresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cellsDepositorInsertHrasls3 (Plaat3 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cas9-T2A-STOP-TdT
Plasmid#126425PurposeExpresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)DepositorInsertCas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
UseTagsExpressionMammalianMutationalanine-6 of TdT has been changed to a STOP codonPromoterpCMVAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-emiRFP703-stop
Plasmid#186386PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP
Plasmid#154303PurposeExpresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideumDepositorInsertshistone H1
γ-tubulin
UseDictyostelium expressionTagsmEGFP and mRuby3ExpressionMutationPromoteractin15Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
p231 pCMVe-betaAc-STOP-luc
Plasmid#12463DepositorInsertCMVe-betaAc-STOP
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-S28 histone 3 phosphorylation reporter
Plasmid#32788DepositorInsertCFP-14-3-3tau-H3 peptide-YFP
UseTagsCFP and YFPExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only