We narrowed to 8,654 results for: pcDNA3

1. pcDNA3.1-SARS2-Spike

   Plasmid

   #145032

   Purpose

   Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 Spike (S Synthetic)
   

   Tags

   C9

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCDNA3-hPXR-FL-T248E

   Plasmid

   #41617

   Depositor

   Sridhar Mani

   Insert

   hPXR-FL-T248E (NR1I2 Human)
   

   Expression

   Mammalian

   Mutation

   N10D and T248E

   Promoter

   CMV

   Available Since

   March 11, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3-hPXR-FL-K277Q

   Plasmid

   #41618

   Depositor

   Sridhar Mani

   Insert

   hPXR-FL-K277Q (NR1I2 Human)
   

   Expression

   Mammalian

   Mutation

   N10D and K277Q

   Promoter

   CMV

   Available Since

   March 11, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3 HA Arf6 ActQ67L

   Plasmid

   #10835

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 Q67L (ARF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q67L. Defective in GTP hydrolysis. Constitutive…

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-2xFLAG-SREBP-1a

   Plasmid

   #26801

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   SREBP-1a (SREBF1 Human)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-N-Flag-NLRP3

   Plasmid

   #75127

   Purpose

   Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-ExRai-AKAR2

   Plasmid

   #161753

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   ExRai-AKAR2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1his p66shc (corrected)

   Plasmid

   #32574

   Depositor

   Toren Finkel

   Article

   Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

   Insert

   p66shc (SHC1 Human)
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-GGamma10-GFP2

   Plasmid

   #166779

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma10-GFP2 (GNG10 Human, Synthetic)
   

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-TLR9-YFP

    Plasmid

    #13642

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 9 (TLR9 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid

    #52421

    Purpose

    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

    Depositor

    Bertrand Collet

    Insert

    Fusion NLS-EGFP-P2A-Cherry-CAAX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2/v5-DEST hGlut4

    Plasmid

    #18087

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    Glut4 (SLC2A4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid

    #212020

    Purpose

    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    UtrCH
    

    Tags

    mStayGold(c4)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Nanoluc-ccdB

    Plasmid

    #87070

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 N-T2A-M-IRES-E

    Plasmid

    #231903

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' system

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, M, E SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    R203M in N protein

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Xl-VSP1 R152Q

    Plasmid

    #51881

    Purpose

    expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 R152Q (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Mutation

    R152Q: near the extracellular side of the fourth …

    Promoter

    CMV

    Available Since

    May 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-(empty)-TAG

    Plasmid

    #138209

    Purpose

    To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Type

    Empty backbone

    Use

    T7 transcription

    Tags

    TAG sequence

    Expression

    Mammalian

    Promoter

    CMV Promoter, T7 Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-GRP78/BiP

    Plasmid

    #32701

    Depositor

    Richard C. Austin

    Article

    Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

    Insert

    GRP78/BiP (HSPA5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-ExRai-AMPKAR(T/A)

    Plasmid

    #192447

    Purpose

    Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR(T/A)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Thr 6 in AMPK substrate mutated to Ala.

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-FLAG-ATBF1

    Plasmid

    #40927

    Depositor

    Jin-Tang Dong

    Article

    Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

    Insert

    ATBF1 (ZFHX3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    CMV

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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