We narrowed to 5,632 results for: chia

1. pAGM22082_cRed

   Plasmid

   #117225

   Purpose

   pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

   Insert

   Canthaxanthin Operon
   

   Use

   Tags

   34 amino acid N-terminal linker, Hexahistidine Ta…

   Expression

   Bacterial

   Mutation

   removed Type IIS restriction sites within the bac…

   Promoter

   T7 RNA Polymerase

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGreenFire_blastocidin

   Plasmid

   #174103

   Purpose

   pGreenFire MPRA plasmid for cloning

   Depositor

   Paul Khavari

   Article

   Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

   Plasmid

   #140018

   Purpose

   Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   EcoTyrRS
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y37L, D182S, F183M, L186A

   Promoter

   EF1

   Available since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

   Plasmid

   #195741

   Purpose

   Base editing for making a pre-stop codon in mmoX using phenol inducible system

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Po (phoenol-inducible promoter)

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB.GFP-KIF1A tail 395-1698

   Plasmid

   #134618

   Purpose

   Mammalian expression of mouse KIF1A 395-1698

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF1A (Kif1a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 394 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CYFIP1.mCherry

   Plasmid

   #122051

   Purpose

   Expresses human CYFIP1 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP1 (CYFIP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBa.GFP-KIF1C tail 400-1103

   Plasmid

   #134621

   Purpose

   Mammalian expression of human KIF1C 400-1103

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF1C (KIF1C Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 399 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUG-T2A-Pten

   Plasmid

   #140738

   Purpose

   Lentiviral vector to overexpress human PTEN and GFP

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-PTA-PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MBP-MEK1

   Plasmid

   #68300

   Purpose

   N-terminal MBP tagged human MEK1 gene for bacterial expression

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   MBP-MEK1 (MAP2K1 Human, Synthetic)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-ITR-EcYtR-mCherry

    Plasmid

    #217362

    Purpose

    Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    E. coli tyrosine tRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

    Plasmid

    #134613

    Purpose

    Mammalian expression of rat KIF5C 378-955

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5C (Kif5c Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE-FKBP-GFP-NDP52

    Plasmid

    #135296

    Purpose

    stably express GFP-tagged NDP52 for CID induced mitophagy

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a-TEX264-deltaLRR-His6

    Plasmid

    #220215

    Purpose

    Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    c.4-75del / p.2-24del

    Promoter

    T7

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL2-Mcl-1

    Plasmid

    #19132

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

    Insert

    Mcl-1 promoter (MCL1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTol2CG2-hsp70I:QFGal4-SV40pA

    Plasmid

    #155120

    Purpose

    Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    hsp70l:QFGal4 (hsp70l Neurospora crassa, Zebrafish, Budding Yeast)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    hsp70l

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

    Plasmid

    #202760

    Purpose

    Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

    Depositor

    Karl Wahlin

    Article

    Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

    Insert

    huAsCpf1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST47-CYFIP1.GFP

    Plasmid

    #109139

    Purpose

    GFP-tagged CYFIP1 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTol2CG2-acta1:QFGal4-SV40pA

    Plasmid

    #155121

    Purpose

    Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    acta1:QFGal4
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    acta1

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA5 TEX264wt-cSSH

    Plasmid

    #220209

    Purpose

    Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNH-TrxT-G-S18

    Plasmid

    #127827

    Purpose

    Production of recombinant human ribosomal protein S18

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS18 (RPS18 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CSII-PPM1H-mApple

    Plasmid

    #198473

    Purpose

    Mammalian expression of PPM1H-mApple

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    PPM1H-mApple (PPM1H Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CSII-PPM1H D288A-mApple

    Plasmid

    #198474

    Purpose

    Mammalian expression of PPM1H-mApple

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    PPM1H-D288A-mApple (PPM1H Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

    Plasmid

    #92228

    Purpose

    Expression of zmADH using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    NTerm 6xHis Tagged zmADH
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNSSCP

    Plasmid

    #183188

    Purpose

    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

    Depositor

    Hsiao-Chun Huang

    Article

    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

    Inserts

    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRCPam

    Plasmid

    #195861

    Purpose

    Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.

    Depositor

    Gregory Phillips

    Article

    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

    Insert

    Amber Chromogenic Protein on pRCPam plasmid
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gln62X

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

    Plasmid

    #134612

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 375 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBa.GFP-KIF13A tail 361-1749

    Plasmid

    #134622

    Purpose

    Mammalian expression of mouse KIF13A 361-1749

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13A (Kif13a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 360 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-pro-siRNA-V2-TP53

    Plasmid

    #111089

    Purpose

    pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Insert

    TP53 (TP53 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBa.GFP-KIF13B tail 442-1843

    Plasmid

    #134623

    Purpose

    Mammalian expression of mouse KIF13B 442-1843

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13B (Kif13b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 441 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc-GFP-dSUMO

    Plasmid

    #85696

    Purpose

    expression of GFP-SUMO1 in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

31. pScalps_Puro_mCebpe

    Plasmid

    #79076

    Purpose

    Expression of mouse Cebpe

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Cebp epsilon (Cebpe Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pPN062

    Plasmid

    #91595

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPN063

    Plasmid

    #91596

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET21b GFP-Rab10 Q68LHis

    Plasmid

    #186015

    Purpose

    GFP-Rab10 Q68LHis

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    GFP-Rab10 Q68LHis (RAB10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDNAIIIB rac1 QL

    Plasmid

    #61633

    Purpose

    mammalian expression of rac1 constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q61L, constitutively active

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pET28a-baPrs-hdNadV

    Plasmid

    #91950

    Purpose

    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Inserts

    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Leucine 135 to Isoleucine

    Promoter

    T7

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUPD2 RDF ( GB1498)

    Plasmid

    #160579

    Purpose

    Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBa.GFP-KIF3B tail 391-747

    Plasmid

    #134615

    Purpose

    Mammalian expression of mouse KIF3B 391-747

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF3B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 390 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBa.FRB-3myc-GS-KIF3A tail 419-702

    Plasmid

    #134614

    Purpose

    Mammalian expression of human KIF3A 419-702

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF3A (KIF3A Human)
    

    Use

    Tags

    FRB, 3myc

    Expression

    Mammalian

    Mutation

    WT with deletion of the 418 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDEST47-CYFIP2.GFP

    Plasmid

    #109140

    Purpose

    GFP-tagged CYFIP2 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBa.GFP-KIF3C tail 411-796

    Plasmid

    #134616

    Purpose

    Mammalian expression of mouse KIF3C 411-796

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF3C (Kif3c Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 410 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pUPD2 PhiC31 integrase (GB1496)

    Plasmid

    #160578

    Purpose

    Streptomyces phage PhiC31 integrase, complete CDS

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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43. pRD408

    Plasmid

    #178199

    Purpose

    Expression of H-NS from its natural promoter

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    phns + hns (hns Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hns promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. pBa.GFP-KIF17 tail 400-1038

    Plasmid

    #134617

    Purpose

    Mammalian expression of mouse KIF1A 400-1038

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF17 (Kif17 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 399 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pBC2T-Ypet-FtnA

    Plasmid

    #108969

    Purpose

    expresses BC2-tag-Ypet-FtnA in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    FtnA (ftnA Escherichia coli)
    

    Use

    Tags

    BC2-tag and Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pNIC28-BSA4-G-S18

    Plasmid

    #127825

    Purpose

    Production of recombinant human ribosomal protein S18

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS18 (RPS18 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pPN098

    Plasmid

    #91625

    Purpose

    Express sgRNA targeting human HCN1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    HCN1 (HCN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. pPN099

    Plasmid

    #91626

    Purpose

    Express sgRNA targeting human HCN1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    HCN1 (HCN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pBEST-p70a-UTR1-Nterm 6xHis PL-T500

    Plasmid

    #92314

    Purpose

    Expression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    B1 domain of protein L (PL)
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pBB0323-TEV-His12

    Plasmid

    #137039

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66700.1
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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