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pC004 - PFS library in Beta-lactamase gene Target Plasmid

Pooled library

#79153

Purpose

Library of protospacer flanking sites (PFS) inserted at the 5' end of the β-lactamase gene for screening PFS preference for Cas13a/C2c2.

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only
pCFD3-MS2tail-Scaffold

Plasmid

#78906

Purpose

Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-pEGFP(isoform 1)

Plasmid

#99561

Purpose

mammalian expression of TMEM230-GFP (isoform 1)

Depositor

Han-Xiang Deng

Publication

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pACYC_HA-ZmSAE1_ZmSAE2-FLAG

Plasmid

#91944

Purpose

Bacterial expression of maize SAE1 and SAE2

Depositor

Richard Vierstra

Publication

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

Plasmid

#91932

Purpose

Bacterial expression of maize SUMO1a and SCE1b

Depositor

Richard Vierstra

Publication

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pWUR Cas2

Plasmid

#80090

Purpose

Encodes Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Insert

Cas2 (cas2 )

Expression

Bacterial

Promoter

T7

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pZS2katGp-RBS33-TP901-proD-oxyR

Plasmid

#78215

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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Mixed-signal integration BAC Reporter

Plasmid

#78223

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
TP901 GFP BAC Reporter

Plasmid

#78216

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
ADC BAC Reporter

Plasmid

#78222

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pZA1pLtetO-GFP

Plasmid

#78227

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pZS1(pLtetO-TP901-aav-proA-tetR)-katGp-PhiC31-proD-oxyR

Plasmid

#78228

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
pZS1katGp-RBS31-PhiC31-proD-oxyR

Plasmid

#78217

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTX201

Plasmid

#89265

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA02

Depositor

Daniel Voytas

Publication

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsYSA-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pTX198

Plasmid

#89262

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

Depositor

Daniel Voytas

Publication

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA01

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pTX168

Plasmid

#89257

Purpose

Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

Depositor

Daniel Voytas

Publication

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET14_GFP5_Encap

Plasmid

#86408

Purpose

Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Publication

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 5aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-chimpTRIM5-FOS

Plasmid

#79065

Purpose

Express chimpanzee TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(chimpanzee)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

Plasmid

#79031

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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