We narrowed to 15,703 results for: gRNA

1. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. L2_lacZgRNA-Cas9-CsA

   Plasmid

   #136138

   Purpose

   Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-CjCas9-MS2gRNA-Backbone

   Plasmid

   #210711

   Purpose

   This plasmid express MS2 loop containing Cj Cas9 gRNA

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MS2 loop containing Cj Cas9 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCAR_sgRNA_puro-mKate-lox5171

   Plasmid

   #162077

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. JAK1 gRNA (BRDN0001145887)

   Plasmid

   #76392

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human JAK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   JAK1 (JAK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. JAK1 gRNA (BRDN0001149025)

   Plasmid

   #76395

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human JAK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   JAK1 (JAK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. ERBB2 gRNA (BRDN0001148320)

   Plasmid

   #76378

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ERBB2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATM gRNA (BRDN0001149518)

   Plasmid

   #77529

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ATM

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ATM (ATM Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRIM28 gRNA (BRDN0001146162)

   Plasmid

   #76142

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. BRCA1 A10.2 gRNA

    Plasmid

    #90549

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA1 (Guide Designation A10.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid

    #74705

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPRv2 EGFP sgRNA2

    Plasmid

    #164933

    Purpose

    Expresses EGFP sgRNA2 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PU6::unc-119_sgRNA

    Plasmid

    #46169

    Purpose

    unc-119 targeting sgRNA

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    unc-119 targeting sgRNA (unc-119 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAT9658-sgRNA-mCherry

    Plasmid

    #162987

    Purpose

    sgRNA cloning plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-P3-evoCjCas9-eAID_gRNA

    Plasmid

    #202562

    Purpose

    P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-eAID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDK2 gRNA (BRDN0001147786)

    Plasmid

    #77192

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK2 (CDK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. STK11 gRNA (BRDN0001146194)

    Plasmid

    #75913

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. gRNA-leu-HYB

    Plasmid

    #64332

    Purpose

    leu2 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of leu2 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. CDK9 gRNA (BRDN0001162576)

    Plasmid

    #77170

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK9

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK9 (CDK9 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. STK11 gRNA (BRDN0001147319)

    Plasmid

    #75914

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge