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pLKO-p53-shRNA-941

Plasmid

#25637

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

p53-shRNA-941 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO-p53-shRNA-941

Plasmid

#25637

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

p53-shRNA-941 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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FCK-Halo-GFP

Plasmid

#14750

Purpose

Lentiviral expression of Halo-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al PLoS One. 2007 Mar 21;2(3):e299.

Insert

Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

N. pharaonis halorhodopsin is mammalian codon-opt…

Promoter

CaMKIIa

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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FCK-Halo-GFP

Plasmid

#14750

Purpose

Lentiviral expression of Halo-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al PLoS One. 2007 Mar 21;2(3):e299.

Insert

Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

N. pharaonis halorhodopsin is mammalian codon-opt…

Promoter

CaMKIIa

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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Dual-sgRNA_hU6-mU6

Plasmid

#154194

Purpose

Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG16_2_synNotch_Gal4VP64

Plasmid

#85421

Purpose

Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

LaG16_2 GFP nanobody synNotch Gal4VP64

Use

Lentiviral

Promoter

PGK

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag Raptor

Plasmid

#26633

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

raptor (RPTOR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag Raptor

Plasmid

#26633

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

raptor (RPTOR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2(1-10)

Plasmid

#82603

Purpose

Lentiviral vector for constitutive expression of sfCherry2(1-10)

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

SFFV

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry3C(1-10)

Plasmid

#117482

Purpose

Lentiviral vector for constitutive expression of sfCherry3C(1-10)

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3C(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

5 substitutions (K45R, G52D, T106A, K182R, N194D)…

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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tetO.Nfib.Hygro

Plasmid

#117271

Purpose

Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Nfib (Nfib Mouse)

Use

Lentiviral

Tags

IRES and hygromycin resistant cassette (C termina…

Expression

Mammalian

Promoter

tet-on

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NICD

Plasmid

#61540

Purpose

Expresses NOTCH-ICD in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

NOTCH-ICD (NOTCH1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

NOTCH intracellular domain (aa1761-2555); A1943S…

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NICD

Plasmid

#61540

Purpose

Expresses NOTCH-ICD in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

NOTCH-ICD (NOTCH1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

NOTCH intracellular domain (aa1761-2555); A1943S…

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUltra-Chili-Luc

Plasmid

#48688

Purpose

3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferase

Depositor

Malcolm Moore

Article

Fujiwara et al Mol Cancer Ther. 2021 Jun 4. pii: 1535-7

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pUltra-Chili-Luc

Plasmid

#48688

Purpose

3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferase

Depositor

Malcolm Moore

Article

Fujiwara et al Mol Cancer Ther. 2021 Jun 4. pii: 1535-7

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-oct4

Plasmid

#20323

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-oct4

Plasmid

#20323

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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tetO.Sox9.Puro

Plasmid

#117269

Purpose

Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

T2A and puromycin resistant cassette (C terminal …

Expression

Mammalian

Promoter

tet-on

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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phASCL1-N106

Plasmid

#31781

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

achaete-scute complex homolog 1 (ASCL1 Human)

Use

Lentiviral

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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phASCL1-N106

Plasmid

#31781

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

achaete-scute complex homolog 1 (ASCL1 Human)

Use

Lentiviral

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge