Skip to main content
Addgene
Showing: 1221 - 1240 of 1266 results

  7. NK85

    Plasmid
    #176611
    Purpose
    To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.
    Depositor
    Meghan Morrissey
    Article
    Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1
    Insert
    FCERG
    Use
    Lentiviral
    Tags
    EGFP and HA, SNAP-tag
    Expression
    Mammalian
    Mutation
    C25A
    Promoter
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176611-plasmid-map-sequence-id-362592

    Enlarge

  9. MLKS2delTMexp

    Plasmid
    #130863
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of transmembrane domain
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130863-plasmid-map-sequence-id-255916

    Enlarge

  11. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

    Enlarge

  12. pCYC1pr-GNSI (LEU)

    Plasmid
    #111450
    Purpose
    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111450-plasmid-map-sequence-id-211605

    Enlarge

  15. KCC2-pHext

    Plasmid
    #104076
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Use
    Tags
    pHluorine tag inserted in the second putative tra…
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104076-plasmid-map-sequence-id-199360

    Enlarge

Showing: 1221 - 1240 of 1266 results