We narrowed to 22,630 results for: CRISPR

1. pKI1.1R

   Plasmid

   #85808

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5A

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCpfSA

   Plasmid

   #187890

   Purpose

   CRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus

   Depositor

   Quanjiang Ji

   Article

   Wang et al ACS Synth Biol. 2022 Sep 16;11(9):3049-3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFREE_Cm

   Plasmid

   #92052

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF-HdrB3

   Plasmid

   #184908

   Purpose

   carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cas9-sgRNA-homology arms
   

   Use

   CRISPR; Broad-host-range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVP141

   Plasmid

   #222079

   Purpose

   Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

   Insert

   dCas9-PmCDA1, sfGFP and sacB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV_Cas9_puro

   Plasmid

   #65655

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   5 ' FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   MSSV_LTR

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRGEB32-BAR

   Plasmid

   #126072

   Purpose

   For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.

   Depositor

   Charles Hunter

   Article

   Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT17

   Plasmid

   #204298

   Purpose

   For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-EYFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSU-araC-Cas9

   Plasmid

   #120423

   Purpose

   Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVT36b

    Plasmid

    #184437

    Purpose

    CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

    Depositor

    Huimin Zhao

    Article

    Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFREE_Amp

    Plasmid

    #92051

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRGE31

    Plasmid

    #50929

    Purpose

    Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter

    Depositor

    Yinong Yang

    Article

    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice snoRNA U3 and dual 35S promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJAT57

    Plasmid

    #204303

    Purpose

    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRPaCas9

    Plasmid

    #111819

    Purpose

    Expresses Cas9 in T. brucei (2T1) cells

    Depositor

    David Horn

    Article

    Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYZ173

    Plasmid

    #98410

    Purpose

    Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ146

    Plasmid

    #98406

    Purpose

    Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.leu1 (leu1 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYZ164

    Plasmid

    #98408

    Purpose

    Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.his3 (his3 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAEF5

    Plasmid

    #136305

    Purpose

    Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

    Depositor

    Gilles Fischer

    Article

    Agier et al Methods Mol Biol. 2021;2196:181-198. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SP6-sgRNA-scaffold

    Plasmid

    #47912

    Purpose

    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

    Depositor

    Paul Sternberg

    Article

    Chiu et al Genetics. 2013 Aug 26.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    sgRNA 3' end

    Expression

    Worm

    Mutation

    Promoter

    SP6

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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