Addgene

We narrowed to 3,333 results for: Ubc

Showing: 1241 - 1260 of 3333 results

Results per page:

Sort results by:

pcDNA3 PM-Ras-LOCKR-PL Key

Plasmid

#216504

Purpose

Ras activity dependent proximity labeler, Key only, plasma membrane localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

PM-Ras-LOCKR-PL Key

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Golgi-Ras-LOCKR-PL Key

Plasmid

#216505

Purpose

Ras activity dependent proximity labeler, Key only, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-PL Key

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 ER-Ras-LOCKR-PL Key

Plasmid

#216506

Purpose

Ras activity dependent proximity labeler, Key only, ER localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

ER-Ras-LOCKR-PL Key

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Mito-Ras-LOCKR-PL Key

Plasmid

#216507

Purpose

Ras activity dependent proximity labeler, Key only, mitochondria localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Mito-Ras-LOCKR-PL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC-N-Venus-V150A-apCC-Di

Plasmid

#190706

Purpose

Encodes a modified version (V150A) of N-Venus followed by the antiparallel coiled-coil apCC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A-apCC-Di

Use

Tags

Expression

Mutation

V150A

Promoter

araBAD

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSub-Asp6-MBP-HRas

Plasmid

#185758

Purpose

Bacterial expression of Asp6-MBP-HRas

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

Asp6-MBP-HRas

Use

Tags

MBP

Expression

Bacterial

Mutation

6x Asp added to the N terminus of MBP

Promoter

tac

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY156: SUZ12 px330 sgRNA Cas9 plasmid

Plasmid

#170792

Purpose

This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6p1 AO7R

Plasmid

#137842

Purpose

GST tagged AO7 encoding aa 126-216 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_MRM1

Plasmid

#111671

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

MRM1 (MRM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-roGFP1-iR

Plasmid

#83314

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iR

Use

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

Promoter

T5

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pCR8-DJ1-E64D

Plasmid

#29356

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Entry vector

Tags

Expression

Mutation

Glu64Asp

Promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pCAGGS_cAMPFIRE-H

Plasmid

#182285

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-H (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV R-CEPIA1er

Plasmid

#58216

Purpose

Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

R-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_cAMPFIRE-M

Plasmid

#182284

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-Mito-TurboID

Plasmid

#200963

Purpose

3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Mito-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cyto-roGFP

Plasmid

#49435

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

roGFP2

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Mito-TurboID

Plasmid

#200960

Purpose

Transiently expresses mitochondria localized TurboID with V5 tags

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

mito-TurboID

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV G-CEPIA1er

Plasmid

#58215

Purpose

Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

G-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Golgi-TurboID

Plasmid

#200959

Purpose

Transiently expresses golgi localized TurboID with HA tags

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-mito-mTagBFP2

Plasmid

#216130

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge