165,817 results

1. Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA

   Pooled Library

   #1000000095

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human genome

   Depositor

   David Sabatini, Eric Lander

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

2. PH-Akt-GFP

   Plasmid

   #51465

   Purpose

   Is a biosensor for PIP3/PI(3,4)P2

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   AKT (aa 1–164)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. CaV2.1 (pcDNA6)

   Plasmid

   #26578

   Depositor

   Diane Lipscombe

   Article

   Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

   Insert

   Cacna1a (Cacna1a Mouse)
   

   Use

   Tags

   His6 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-hM4D(Gi)-mCherry (AAV Retrograde)

   Viral Prep

   #50475-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. CNO-induced neuronal silencing. Synapsin-driven with mCherry reporter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

5. BRAF in pENTR

   Plasmid

   #216605

   Purpose

   gateway cloning donor vector containing BRAF

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   BRAF (BRAF Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1_CsR-Ck1

   Plasmid

   #136124

   Purpose

   L1 in position 1 (next to LB in L2 construct), for CsR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35Sx2:mALS_3t35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. qTAG-C-miRFP670nano3-Blast

   Plasmid

   #207713

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDEST-CMV mCherry-GFP-LC3B WT

   Plasmid

   #123230

   Purpose

   Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human CRISPR Knockout Pooled Library (H3)

   Pooled Library

   #133914

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human genome - version H3

   Depositor

   Myles Brown, Xiaole Shirley Liu

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pLKO-p53-shRNA-941

    Plasmid

    #25637

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-941 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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