163,421 results

1. CAG-Crx-IRES-GFPd2

   Plasmid

   #73997

   Purpose

   Plasmid expressing Crx-IRES- GFPd2

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   Crx-IRES-GFPd2 (Crx Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-SFFV-KRAB-dCas9-P2A-mCherry

   Plasmid

   #60954

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Insert

   KRAB-dCas9-P2A-mCherry fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, KRAB domain, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Plxna1-Fc-His

   Plasmid

   #72122

   Purpose

   Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxna1 (Plxna1 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-HA-USP20

   Plasmid

   #22573

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP20 (USP20 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 phiC31

   Plasmid

   #68310

   Purpose

   In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

   Depositor

   Konrad Basler

   Article

   Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

   Insert

   phiC31
   

   Use

   Rna transcription

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pP2A-mCherry-N1

   Plasmid

   #84329

   Purpose

   Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   P2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. minCMV-1xmNG-dMCP

   Plasmid

   #231091

   Purpose

   Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).

   Depositor

   John Ngo

   Insert

   1xmNG-dMCP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRTC2-puro

   Plasmid

   #190567

   Purpose

   Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.

   Depositor

   Anthony Furano

   Article

   Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   L1 (L1.3) with reporter cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFLAG-SURF4

    Plasmid

    #170680

    Purpose

    Expresses FLAG-tagged SURF4 in mammalian cells.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    SURF4
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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