Addgene

We narrowed to 9,674 results for: Ada;

Showing: 1261 - 1280 of 9674 results

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cfmTurquoise-N

Plasmid

#139284

Purpose

Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

Depositor

Ikuo Wada

Article

Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

Insert

cfmTurquoise

Use

Tags

Multi-cloning sites

Expression

Mammalian

Mutation

Cys48Ser and Cys70/Met

Promoter

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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CABE-T2.9

Plasmid

#193264

Purpose

Expression of CABE-T2.9 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T2.9

Use

Tags

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MEN2A-V5

Plasmid

#139629

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2A (RET Human)

Use

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

C634W

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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MEN2B-V5

Plasmid

#139630

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2B (RET Human)

Use

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

M918T

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE-POLArISact

Plasmid

#164970

Purpose

T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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FN 108

Plasmid

#50495

Depositor

Kenneth Yamada

Article

Aota et al J Biol Chem. 1994 Oct 7;269(40):24756-61

Insert

fibronectin (FN1 Human)

Use

Tags

Expression

Bacterial

Mutation

repeats 9 and 10; EcoR1 site on III-9 is mutated,…

Promoter

T7

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
RGS-6xHis-mRFP-pcDNA3.1-

Plasmid

#52516

Purpose

expresses mRFP in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

mRFP

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

engineered variant of tetrameric red fluorescent …

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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PTPRA_C433S-GFP fusion

Plasmid

#139638

Purpose

GFP-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA_C433S (PTPRA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C433S

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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PTPRA_C723S-GFP fusion

Plasmid

#139639

Purpose

GFP-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA_C723S (PTPRA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C723S

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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RET_KD-V5

Plasmid

#139628

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET KD (RET Human)

Use

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

K758R

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-ONL

Plasmid

#65713

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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CABE-T2.6

Plasmid

#193263

Purpose

Expression of CABE-T2.6 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T2.6

Use

Tags

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.6

Plasmid

#193275

Purpose

Expression of CBE-T1.6 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.6

Use

Tags

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170816

Purpose

Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170817

Purpose

Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mNeonGreen-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pmKikGR Vinculin

Plasmid

#105316

Purpose

Photoconvertable focal adhesion protein vinculin to study kinetics or dynamics

Depositor

Kenneth Yamada

Article

Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

Insert

vinculin (VCL Chicken)

Use

Tags

KikGR

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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RET_Y1062F-SHC

Plasmid

#139620

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET_Y1062F (RET Human)

Use

Tags

StrepIII-HA

Expression

Mammalian

Mutation

Y1062F

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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3604 pGEX4T-1 Bad S136A (104-141)

Plasmid

#8801

Depositor

Stanley Korsmeyer

Article

Harada et al Mol Cell 1999 Apr;3(4):413-22.

Insert

Bad (Bad Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains AA's 104-141; S136A

Promoter

Ptac

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)_UG3

Plasmid

#133874

Purpose

Expresses UG3 in mammalian cells.

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH (UTRN Human)

Use

Tags

helical linker - mEGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-DRE-1-pcDNA3.1-

Plasmid

#52517

Purpose

expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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