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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,972 results for: nts

Showing: 1261 - 1280 of 15972 results

  1. pYPQAT37

    Plasmid
    #223409
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223409-plasmid-map-sequence-id-453514

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  2. pYPQAT40

    Plasmid
    #223412
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223412-plasmid-map-sequence-id-453531

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  4. pYPQAT56

    Plasmid
    #223428
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223428-plasmid-map-sequence-id-453528

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  5. pYPQ131-tRNA2.0

    Plasmid
    #158393
    Purpose
    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158393-plasmid-map-sequence-id-330365

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  11. pRGEB32-BAR

    Plasmid
    #126072
    Purpose
    For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.
    Depositor
    Charles Hunter
    Article
    Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126072-plasmid-map-sequence-id-247528

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  12. pYPQ132-tRNA2.0

    Plasmid
    #158394
    Purpose
    Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158394-plasmid-map-sequence-id-330366

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  13. pYPQAT59

    Plasmid
    #223431
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223431-plasmid-map-sequence-id-453576

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  15. pYPQ133-tRNA2.0

    Plasmid
    #158395
    Purpose
    Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158395-plasmid-map-sequence-id-330367

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  16. pYPQ134-tRNA2.0

    Plasmid
    #158396
    Purpose
    Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158396-plasmid-map-sequence-id-330816

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  17. pYPQ135-tRNA2.0

    Plasmid
    #158397
    Purpose
    Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158397-plasmid-map-sequence-id-330905

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  18. pYPQ136-tRNA2.0

    Plasmid
    #158398
    Purpose
    Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158398-plasmid-map-sequence-id-330906

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Showing: 1261 - 1280 of 15972 results