We narrowed to 15,783 results for: LEA

1. pX330-Flag-dSpCas9-HF1

   Plasmid

   #92115

   Purpose

   Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-deSpCas9-VP64-6xHis

   Plasmid

   #92117

   Purpose

   Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. hFLT1 delta CTD

   Plasmid

   #86036

   Purpose

   human VEGFR1 with deletion of the entire intracellular domain

   Depositor

   Christie Thomas

   Article

   Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

   Insert

   human Flt1 delta CTD (FLT1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   deta CTD

   Promoter

   CMV

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-mCherry-TSC2(AA)-NLS

   Plasmid

   #192443

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HeFSpCas9

   Plasmid

   #92355

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330-Flag-eSpCas9 (without sgRNA)

   Plasmid

   #92354

   Purpose

   Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-dHeFSpCas9-VP64-6xHis

   Plasmid

   #92119

   Purpose

   Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNUT N6His K206E hTFNG

   Plasmid

   #70130

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   He et al Biochemistry. 1999 Jul 27;38(30):9704-11

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   changed Lys206 to a glutamic acid, Asn413Asp, Asn…

   Promoter

   SV40

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-GRP78-DeltaER

    Plasmid

    #238416

    Purpose

    Expresses GRP78 lacking the ER signal peptide in mammalian cells

    Depositor

    Amy Shiu Lee

    Article

    Liu et al J Biol Chem. 2025 Feb 28;301(4):108369.

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The first 18 amino acids which is the ER signal p…

    Promoter

    Available since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+-FLAG3-drAgo2D-A

    Plasmid

    #221713

    Purpose

    Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2D-A (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    D683A in active site

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-FLAG3-drAgo2DY-EF

    Plasmid

    #221733

    Purpose

    Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2DY-EF (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D651E and Y680F in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2+-FLAG3-hsAgo2F-Y

    Plasmid

    #221722

    Purpose

    Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2F-Y (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    F666Y in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+-FLAG3-hsAgo2E-D

    Plasmid

    #221721

    Purpose

    Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2E-D (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    E637D in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2+-FLAG3-hsAgo2D-A

    Plasmid

    #221720

    Purpose

    Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2D-A (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    D669A in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2+-FLAG3-hsAgo2

    Plasmid

    #221719

    Purpose

    Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2 (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2+-FLAG3-drAgo2Y-F

    Plasmid

    #221715

    Purpose

    Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2Y-F (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Y680F in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+-FLAG3-drAgo2D-E

    Plasmid

    #221714

    Purpose

    Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2D-E (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    D651E in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBS-859

    Plasmid

    #218009

    Purpose

    mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Tags

    Factor Xa protease cleavage site and Strep-tag II

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HeFm1SpCas9

    Plasmid

    #92110

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge