We narrowed to 4,158 results for: SPL

Showing: 1281 - 1300 of 4158 results

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pJSM103

Plasmid

#48872

Purpose

NHEJ reporter plasmid for blunt ends

Depositor

Michael Glickman

Article

Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

Insert

split lacZ

Expression

Bacterial

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-D2irG4kwh

Plasmid

#44723

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
EGFP

Use

Synthetic Biology; Expression regulator/reporter

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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FKBP_cPPVp

Plasmid

#118967

Purpose

Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cPPVp in fusion with FKBP

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1815_pCR8-gSMN2-DN3

Plasmid

#118648

Purpose

Transient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRAM29

Plasmid

#234315

Purpose

G264A Cat-RNA U1367, Catalytically dead ribozyme control

Depositor

James Chappell

Article

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

Splicing-deficient mutant of ribozyme

Use

Synthetic Biology

Promoter

P. CymRC

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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ABI_cPPVp

Plasmid

#119212

Purpose

Expresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cPPVp in fusion with ABI

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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sZF4

Plasmid

#43835

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF4

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pMMDuet19

Plasmid

#45594

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

NpuDnaB mini intein

Tags

GB1 and H6-GB1

Expression

Bacterial

Mutation

removal of endonuclease domain

Promoter

T7

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

Plasmid

#229770

Purpose

Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

Depositor

Hirofumi Kai

Article

Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

Insert

Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)

Use

Luciferase

Tags

LgBiT

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

Plasmid

#229769

Purpose

Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

Depositor

Hirofumi Kai

Article

Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

Insert

Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)

Use

Luciferase

Tags

SmBiT

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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P2_HNF4A8

Plasmid

#31094

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 8 derived from promoter P2

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-dCasRx-ccdB

Plasmid

#221003

Purpose

Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_C

Plasmid

#209554

Purpose

pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

2xFKBP-TadA-8e(77-167)-SpCas9(D10A)

Expression

Mammalian

Promoter

pCMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTopo pLox hPGK-Puro pA pLox

Plasmid

#171048

Purpose

Empty donor template for CRISPR/Cas9 targeting of TERT RE's

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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PYL1_nPPVp

Plasmid

#119211

Purpose

Expresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nPPVp in fusion with PYL1

Tags

AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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FRB_nPPVp

Plasmid

#118966

Purpose

Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nPPVp in fusion with FRB

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PLL7.0 tgRFP cPYP

Plasmid

#114464

Purpose

Mammalian expression of tgRFP labelled cPYP

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

cPYP

Tags

tgRFP

Expression

Mammalian

Mutation

M121E

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA RBD-GFP11

Plasmid

#133069

Purpose

Expresses RBD domain with tripartite split GFP11 (M4)

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

RBD (Rhotekin-Rho Binding Domain)

Tags

GFP11 (tripartite split-GFP)

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-hSyn-IntCCCre-T2A-CopGFP

Plasmid

#160503

Purpose

Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

IntCCCre

Use

Cre/Lox and Lentiviral

Tags

CopGFP

Expression

Mammalian

Promoter

hSyn

Available Since

March 12, 2022

Availability

Academic Institutions and Nonprofits only

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